NEURAL CONTROL OF MAGNOCELLULAR NEUROENDOCRINE CELLS

巨细胞神经内分泌细胞的神经控制

• 批准号: 3397247
• 负责人: GLENN I HATTON
• 金额: $ 13万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-01-01 至 1991-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3397247
• 关键词: afferent nerve; electrophysiology; enkephalins; gap junctions; histochemistry /cytochemistry; hypothalamus; immunochemistry; laboratory rat; membrane structure; neural information processing; neural inhibition; neural initiation; neural transmission; neuroanatomy; neuroendocrine system; neuropeptides; oxytocin; synapses; vasopressins

The long-range goal of our research is to understand the mechanisms involved in the control of hormone secretion from the magnocellular neuroendocrine cells (MNCs) whose cell bodies reside predominantly in the supraoptic (SON) and paraventricular (PVN) nuclei of the hypothalamus. Since the patterns of electrical activity are dominant factors in the process of neurosecretion by these cells, our immediate goals for the research proposed here are to investigate some of the factors which may influence or determine their activity patterns. In particular we wish to focus on investigating membrane properties, synaptic inputs and non-synaptic interactions, all of which probably play roles in controlling or modulating the process of neurosecretion in this system. In order to accomplish these objectives, we will record intracellular from PVN and perifornical neurons and from both neurons and glial cells in the area of the SON in slices of rat hypothalamus. In some instances the electrodes will be filled with the fluorescent dye, Lucifer Yellow (LY), and in others with horseradish peroxidase (HRP) in order to identify the recorded cell and trace its processes. Cells marked with LY will undergo either subsequent immunocytochemical analysis of hormone content (oxytocin, vasopressin or enkephalin) or electron microscopic (EM) analysis to demonstrate gap junctions after rendering the LY electron dense. HRP-injected cells and their processes will be analyzed at both the light and EM levels in order to determine the collateral branching and axonal terminations of these neurons. Studies of putative transmitters will also be carried out. Thus, we will be able to determine the physiological, morphological and hormonal characteristics of cells in this brain area, as well as to learn more about the local circuitry in which these cell groups apparently participate.

我们研究的长期目标是了解其机制