ELECTROMAGNETIC FIELD STIMULATION OF NERVE REGENERATION

电磁场刺激神经再生

基本信息

批准号：
2267733
负责人：
BETTY FLORIO SISKEN
金额：
$ 9.47万
依托单位：
UNIVERSITY OF KENTUCKY
依托单位国家：
美国
项目类别：
财政年份：
1991
资助国家：
美国
起止时间：
1991-08-01 至 1995-06-30
项目状态：
已结题

项目摘要

Severe trauma to peripheral nerves often results in long-term disability and loss of personal autonomy. The number of persons suffering from nerve injuries is approximately 1 million per year. Enhancing recovery from such injuries would obviously be of utmost benefit. One of the few techniques that can be applied easily and non-invasively to accelerate the healing process involves the use of pulsed electromagnetic fields (PEMF). In our laboratory we have observed a statistically significant 22% increase in the rate of regeneration when PEMF is applied after crush lesion of rat sciatic nerve. This enhancement is comparable to that achieved by drugs, growth factors or surgical "conditioning lesions." If applied to the animal before the nerve injury, we also observed a heightened regenerative response. Our objective in these studies is to determine the mechanisms underlying the promotion of increased nerve regeneration by PEMF in an animal model. The mechanisms to be addressed are: (1) modification of slow axonal transport, (2) upregulation of RNA and protein synthesis in neuronal cells for the regrowth of nerve fibers, (3) augmentation of synthesis of mitogens and trophic factors from cells at the site of injury and in the distal segment, and (4) interaction with exogenous nerve growth factor to promote cell survival. Lastly, we will address these mechanistic questions on parallel cell culture models treated with the electromagnetic fields.

对周围神经的严重创伤常常导致长期残疾和丧失个人自主权。遭受神经的人数伤害每年约100万。增强这种恢复伤害显然将是最大的好处。为数不多的技术之一可以轻松，无创地应用以加速愈合过程涉及使用脉冲电磁场（PEMF）。在我们的实验室我们观察到统计学上显着的22％大鼠坐骨神经病变施用PEMF时再生速率神经。这种增强与药物实现的增强相当因素或手术“调理病变”。如果应用于动物在神经损伤之前，我们还观察到增长的再生回复。我们在这些研究中的目标是确定机制 PEMF在动物模型。要解决的机制是：（1）修改缓慢的轴突运输，（2）RNA和蛋白质合成中的上调神经纤维再生的神经元细胞，（3）增强损伤部位的细胞中有丝分裂因子和营养因子的合成在远端段，（4）与外源性神经生长的相互作用促进细胞存活的因素。最后，我们将解决这些机制关于用电磁处理的平行细胞培养模型的问题字段。