ELECTROMAGNETIC FIELD STIMULATION OF NERVE REGENERATION

电磁场刺激神经再生

• 批准号: 2267733
• 负责人: BETTY FLORIO SISKEN
• 金额: $ 9.47万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 1995-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2267733
• 关键词: RNA biosynthesis; Schwann cells; actins; antibody; autoradiography; biological models; chick embryo; cytoskeletal proteins; electromagnetic radiation; fibroblasts; growth factor receptors; histology; immunocytochemistry; laboratory rat; leucine; mitogens; morphology; nervous system regeneration; neurofilament; neuronal transport; neurons; neurotrophic factors; peripheral nervous system; protein biosynthesis; thymidine; tissue /cell culture; trauma; tritium; uridine

Severe trauma to peripheral nerves often results in long-term disability and loss of personal autonomy. The number of persons suffering from nerve injuries is approximately 1 million per year. Enhancing recovery from such injuries would obviously be of utmost benefit. One of the few techniques that can be applied easily and non-invasively to accelerate the healing process involves the use of pulsed electromagnetic fields (PEMF). In our laboratory we have observed a statistically significant 22% increase in the rate of regeneration when PEMF is applied after crush lesion of rat sciatic nerve. This enhancement is comparable to that achieved by drugs, growth factors or surgical "conditioning lesions." If applied to the animal before the nerve injury, we also observed a heightened regenerative response. Our objective in these studies is to determine the mechanisms underlying the promotion of increased nerve regeneration by PEMF in an animal model. The mechanisms to be addressed are: (1) modification of slow axonal transport, (2) upregulation of RNA and protein synthesis in neuronal cells for the regrowth of nerve fibers, (3) augmentation of synthesis of mitogens and trophic factors from cells at the site of injury and in the distal segment, and (4) interaction with exogenous nerve growth factor to promote cell survival. Lastly, we will address these mechanistic questions on parallel cell culture models treated with the electromagnetic fields.

严重的周围神经损伤往往导致长期残疾 丧失个人自主权。 患有神经衰弱的人数 每年约有100万人受伤。 加强从这种 受伤显然是最大的好处。 为数不多的技术之一 可以简单无创地加速伤口愈合 该过程涉及使用脉冲电磁场（PEMF）。 在我们 我们在实验室观察到， 大鼠坐骨神经挤压伤后应用PEMF的再生率 神经。 这种增强与药物、生长 因素或外科“条件性病变。“如果应用于动物 在神经损伤之前，我们还观察到 反应 我们在这些研究中的目标是确定 潜在的促进增加神经再生的PEMF中， 动物模型 要解决的机制是：（1）修改 慢轴突运输，（2）RNA和蛋白质合成的上调， 神经元细胞用于神经纤维的再生，（3）增强 从损伤部位的细胞合成有丝分裂原和营养因子 和远端段;（4）与外源性神经生长的相互作用 促进细胞存活的因子。 最后，我们将讨论这些机制 关于电磁波处理的平行细胞培养模型的问题 领域的

项目成果

Acute treatment with pulsed electromagnetic fields and its effect on fast axonal transport in normal and regenerating nerve.

脉冲电磁场​​的急性治疗及其对正常和再生神经快速轴突运输的影响。

• DOI: 10.1002/jnr.490420512
• 发表时间: 1995
• 期刊: Journal of neuroscience research.
• 作者: Sisken,BF;Jacob,JM;Walker,JL
• 通讯作者: Walker,JL