ELECTROMAGNETIC FIELD STIMULATION OF NERVE REGENERATION

电磁场刺激神经再生

• 批准号: 3416465
• 负责人: BETTY FLORIO SISKEN
• 金额: $ 9.07万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 1994-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3416465
• 关键词: RNA biosynthesis; Schwann cells; actins; antibody; autoradiography; biological models; chick embryo; cytoskeletal proteins; electromagnetic radiation; fibroblasts; growth factor receptors; histology; immunocytochemistry; laboratory rat; leucine; mitogens; morphology; nervous system regeneration; neurofilament; neuronal transport; neurons; neurotrophic factors; peripheral nervous system; protein biosynthesis; thymidine; tissue /cell culture; trauma; tritium; uridine

Severe trauma to peripheral nerves often results in long-term disability and loss of personal autonomy. The number of persons suffering from nerve injuries is approximately 1 million per year. Enhancing recovery from such injuries would obviously be of utmost benefit. One of the few techniques that can be applied easily and non-invasively to accelerate the healing process involves the use of pulsed electromagnetic fields (PEMF). In our laboratory we have observed a statistically significant 22% increase in the rate of regeneration when PEMF is applied after crush lesion of rat sciatic nerve. This enhancement is comparable to that achieved by drugs, growth factors or surgical "conditioning lesions." If applied to the animal before the nerve injury, we also observed a heightened regenerative response. Our objective in these studies is to determine the mechanisms underlying the promotion of increased nerve regeneration by PEMF in an animal model. The mechanisms to be addressed are: (1) modification of slow axonal transport, (2) upregulation of RNA and protein synthesis in neuronal cells for the regrowth of nerve fibers, (3) augmentation of synthesis of mitogens and trophic factors from cells at the site of injury and in the distal segment, and (4) interaction with exogenous nerve growth factor to promote cell survival. Lastly, we will address these mechanistic questions on parallel cell culture models treated with the electromagnetic fields.

周围神经的严重创伤往往会导致长期的残疾 以及个人自主权的丧失。患有神经症的人数 每年大约有100万人受伤。加强从这种情况下恢复 伤病显然是最大的好处。为数不多的几种技术之一 可以方便地、非侵入性地使用以加速愈合 该过程涉及到脉冲电磁场(PEMF)的使用。在我们的 我们观察到在统计上显著增加了22%的 大鼠坐骨神经挤压伤后应用PEMF的再生率 很有胆量。这种增强可以与药物、生长 因素或外科“条件性损伤”。如果应用于动物 在神经损伤前，我们还观察到了高度的再生 回应。我们在这些研究中的目标是确定 PEMF促进小鼠神经再生的机制 动物模型。需要解决的机制是：(1)修改 轴突运输缓慢，(2)RNA和蛋白质合成上调 神经纤维再生的神经细胞，(3)增强 损伤部位细胞合成有丝分裂原和营养因子 (4)与外源性神经生长的相互作用 促进细胞存活的因子。最后，我们将解决这些机械性问题 关于电磁处理平行细胞培养模型的几个问题 菲尔兹。