IDENTIFICATION AND PHARMACODYNAMICS OF LIPOPHILIC LIGAND
IDENTIFICATION AND PHARMACODYNAMICS OF LIPOPHILIC LIGAND
批准号:
3440918
负责人:
HAROLD L KOMISKEY
金额:
$8.58万
依托单位国家:
美国
项目类别:
财政年份:
1989
资助国家:
美国
项目状态:
已结题
起止时间:
1989-05-01 至 1992-04-30
中文摘要
一种对中枢具有高亲和力的亲脂物质
英文摘要
A lipophilic substance with high affinity for central
benzodiazepine receptors has been extracted from rat brain tissue.
The lipophilic substance reduces gamma-aminobutyric acid-stimulated
uptake of chloride in synaptoneurosomes. In preliminary studies
the benzodiazepine antagonist, flumazepil, blocked the inhibitory
effect of the lipophilic substance on gamma-aminobutyric acid-
stimulated chloride uptake. The lipophilic substance does not
appear to be a benzodiazepine, a beta-carboline or a polypeptide.
The objectives of this grant should help explain why humans vary
in response to the benzodiazepines. The first specific aim is to
obtain the lipophilic substance in 95 to 100 purity. State of the
art spectroscopic equipment will be used to determine the structure
of the lipophilic substance. The affinity of the lipophilic
substance for central and peripheral benzodiazepine receptors will
be assessed by its ability to displace -H-flumazepil and -H-PK11195
specific binding, respectively. The selectivity of the lipophilic
substance for benzodiazepine receptors will be determined by its
ability to displace the specific binding of radio-ligands with
selectivity for other receptors. The potency of the lipophilic
compound in decreasing muscimol-stimulated chloride uptake in
synaptoneurosomes will be measured. To control for stress induced
alterations of chloride transport, a handled-habituated group will
be included in these latter studies. The mechanism by which the
lipophilic substance initiates the decrease in muscimol-stimulated
chloride uptake will be clarified. Flumazepil, PK11195 and Ro15-
4513 will be examined for an ability to antagonize the reduction
in muscimol-stimulated chloride uptake elicited by the lipophilic
substance.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
LONG ACTING ANTICOCAINE THEAPY
-
批准号:6318330
-
项目类别:
-
资助金额:$5.71万
-
财政年份:2000
-
负责人:HAROLD L KOMISKEY
-
依托单位:
MIRDP AT XAVIER UNIVERSITY OF LOUISIANA
-
批准号:3450347
-
项目类别:
-
资助金额:$13.69万
-
财政年份:1992
-
负责人:HAROLD L KOMISKEY
-
依托单位:
MIDARP AT XAVIER UNIVERSITY OF LOUISIANA
-
批准号:6378555
-
项目类别:
-
资助金额:$40.61万
-
财政年份:1992
-
负责人:HAROLD L KOMISKEY
-
依托单位:
LONG ACTING ANTICOCAINE THEAPY
-
批准号:6205126
-
项目类别:
-
资助金额:$5.71万
-
财政年份:1992
-
负责人:HAROLD L KOMISKEY
-
依托单位:
MIRDP
-
批准号:2120411
-
项目类别:
-
资助金额:$45.63万
-
财政年份:1992
-
负责人:HAROLD L KOMISKEY
-
依托单位:
MIRDP AT XAVIER UNIVERSITY OF LOUISIANA
-
批准号:3450348
-
项目类别:
-
资助金额:$33.88万
-
财政年份:1992
-
负责人:HAROLD L KOMISKEY
-
依托单位:
MIDARP AT XAVIER UNIVERSITY OF LOUISIANA
-
批准号:2840405
-
项目类别:
-
资助金额:$40.0万
-
财政年份:1992
-
负责人:HAROLD L KOMISKEY
-
依托单位:
MIRDP AT XAVIER UNIVERSITY OF LOUISIANA
-
批准号:2120410
-
项目类别:
-
资助金额:$47.87万
-
财政年份:1992
-
负责人:HAROLD L KOMISKEY
-
依托单位:
MIRDP AT XAVIER UNIVERSITY OF LOUISIANA
-
批准号:2120412
-
项目类别:
-
资助金额:$46.36万
-
财政年份:1992
-
负责人:HAROLD L KOMISKEY
-
依托单位:
MIRDP AT XAVIER UNIVERSITY OF LOUISIANA
-
批准号:2458394
-
项目类别:
-
资助金额:$41.77万
-
财政年份:1992
-
负责人:HAROLD L KOMISKEY
-
依托单位:
MIDARP AT XAVIER UNIVERSITY OF LOUISIANA
-
批准号:6175649
-
项目类别:
-
资助金额:$39.35万
-
财政年份:1992
-
负责人:HAROLD L KOMISKEY
-
依托单位:
MIRDP AT XAVIER UNIVERSITY OF LOUISIANA
-
批准号:3450346
-
项目类别:
-
资助金额:$35.0万
-
财政年份:1992
-
负责人:HAROLD L KOMISKEY
-
依托单位:
IDENTIFICATION AND PHARMACODYNAMICS OF LIPOPHILIC LIGAND
-
批准号:3440917
-
项目类别:
-
资助金额:$0.49万
-
财政年份:1989
-
负责人:HAROLD L KOMISKEY
-
依托单位:
IDENTIFICATION AND PHARMACODYNAMICS OF LIPOPHILIC LIGAND
-
批准号:3838808
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:HAROLD L KOMISKEY
-
依托单位:
IDENTIFICATION AND PHARMACODYNAMICS OF LIPOPHILIC LIGAND
-
批准号:3753063
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:HAROLD L KOMISKEY
-
依托单位:
IDENTIFICATION AND PHARMACODYNAMICS OF LIPOPHILIC LIGAND
-
批准号:3775222
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:HAROLD L KOMISKEY
-
依托单位:
海外基金