GENETICS OF SUBSTITUTED AROMATIC COMPOUND METABOLISM

土壤细菌的分解代谢基因提供了一个独特的研究体系 原核基因调控的基本过程。 主要 本研究的目的是探讨在一个池的分歧， 编码取代芳香化合物的结构基因 猫 我们建议确定并澄清以下方面的作用： 酶战略性地位于代谢途径中， 细菌宿主的表型能力。 我们会研究 这些战略酶的贡献，利用 化石、可再生和合成化合物。 这些研究将 也使我们能够将这些细胞的结构和功能 内切酶 为此，基因的结构和调控 编码氯儿茶酚双加氧酶和单加氧酶的基因将被 分析了 我们感兴趣的两种单加氧酶是 水杨酸羟化酶和2，4-D单加氧酶。 另一个目标是研究细菌基因， 控制取代芳族化合物氧化的分解代谢途径 化合物转化为三羧酸循环中间体， 互补、重组DNA和其它技术。 监管 控制氯代芳香族催化剂的基因，tfdR/S和tfdT 2，4-二氯苯氧基乙酸酯（2，4-D）和1，4-二氯苯的dcbR 降解，将在分子水平上进行剖析。 的作用 由三个2，4-D调控基因编码的蛋白质中， 考察 第三个目标是探讨其作用机制 单个和多个调控元件和分子细节 的会聚途径的部分表达，2，4-D和 1，4-二氯苯催化剂

Catabolic genes of soil bacteria offer a unique system for studying fundamental processes of procaryotic gene regulation. The main objective of this research is to explore the divergence in a pool of structural genes encoding substituted aromatic compound catabolism. We propose to characterize and clarify the role of enzymes strategically located in a metabolic route in extending the phenotypic capabilities of bacterial hosts. We will examine the contribution of these strategic enzymes to the utilization of fossil, renewable, and synthetic compounds. These studies will also enable us to correlate the structure and function of these enzymes. To this end, the structure and regulation of genes encoding chlorocatechol dioxygenases and monooxygenases will be analyzed. Two types of monooxygenases of our interest are salicylate hydroxylase and 2,4-D monooxygenase. Another objective is to study bacterial genes that regulate catabolic pathways governing the oxidation of substituted aromatic compounds to tricarboxylic acid cycle intermediates using complementation, recombinant DNA and other techniques. Regulatory genes controlling chloroaromatic catabolism, tfdR/S and tfdT of 2,4-dichlorophenoxyacetate (2,4-D) and dcbR of 1,4-dichlorobenzene degradation, will be dissected at the molecular level. The role of the proteins encoded by three 2,4-D regulatory genes will be examined. The third objective is to investigate the action mechanisms of single and multiple regulatory elements and the molecular details of the partial expression of convergent pathways for 2,4-D and 1,4-dichlorobenzene catabolism.