NEUROMUSCULAR TRANSMISSION IN URINARY SMOOTH MUSCLE

泌尿平滑肌中的神经肌肉传递

• 批准号: 3462637
• 负责人: JACEK MOSTWIN
• 金额: $ 10.16万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-08-01 至 1991-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3462637
• 关键词: electrophysiology; electrostimulus; enzyme mechanism; guinea pigs; human tissue; microelectrodes; neural transmission; neuromuscular function; smooth muscle; stimulus /response; urinary bladder

A proposal is presented to establish a laboratory to study the cellular properties of neuromuscular transmission in the smooth muscle of the three major functional areas of the human lower urinary tract using intracellular microelectrodes, tension recording, and classical pharmacological intervention. Tissue will be obtained from adults and children with normal and abnormal urinary muscle function undergoing open urologic operations. Experiments will be designed to examine the mechanical and electrical responses of muscle strips to nerve stimulation and to identify the neurotransmitters responsible by reproducing and abolishing the response with selected agonists and antagonists. The contribution of adenosine 5'-triphosphate to non-adrenergic non-cholinergic transmission will be particularly examined because growing evidence supports the role of this compound in such transmission in several adrenergic and cholinergic systems. Changes in membrane excitability as manifested by changes in the resting membrane potential and the response to stimulation by agonists, depolarizing solutions and electrical stimulation will be examined in normal and abnormal urinary muscle because preliminary evidence suggests that such changes may be implicated in pathologically abnormal urinary muscle associated with clinical motility disorders. It is hoped that the work resulting from this proposal will contribute to existing knowledge of normal cellular events associated with voiding, will identify significant similarities and differences among the functional regions of the urinary tract, and will contribute toward understanding the extent to which changes in the cellular mechanisms of urinary tract smooth muscle contribute to clinical disorders of urinary tract motility. The applicant is a qualified clinical urologist with three years experience in the laboratory study of Smooth Muscle. This award would constitute a major opportunity for the applicant to develop his research and clinical goals, to establish an independent laboratory to investigate basic mechanism of urinary tract smooth muscle while continuing his clinical work with patients with neuromuscular bladder disorders.

提议建立一个实验室来研究细胞 三种平滑肌的神经肌肉传递特性 使用细胞内的人类下尿路的主要功能区域 微电极、张力记录和经典药理学 干涉。 组织将从正常的成人和儿童处获得 以及接受开放泌尿外科手术的泌尿肌功能异常。 实验将被设计来检查机械和电气 肌肉条对神经刺激的反应并识别 神经递质负责复制和消除反应 与选定的激动剂和拮抗剂。 腺苷的贡献 5'-三磷酸至非肾上腺素能非胆碱能传递将是 特别受到审查，因为越来越多的证据支持这一作用 化合物在几种肾上腺素能和胆碱能的这种传输中 系统。 膜兴奋性的变化表现为 静息膜电位和对激动剂刺激的反应， 去极化溶液和电刺激将在 正常和异常的泌尿肌，因为初步证据表明 这些变化可能与病理性异常尿有关 与临床运动障碍相关的肌肉。 希望 该提案所产生的工作将有助于丰富现有知识 与排尿相关的正常细胞事件将识别出显着的 泌尿系统功能区之间的异同 道，并将有助于理解变化的程度 尿路平滑肌的细胞机制有助于 尿路运动的临床障碍。 申请人是具有三年经验的合格临床泌尿科医生 在平滑肌的实验室研究中。 该奖项将构成 申请人发展其研究和临床的重大机会 目标是建立一个独立的实验室来研究基础 在继续临床工作的同时研究泌尿道平滑肌的机制 患有神经肌肉膀胱疾病的患者。