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MOLECULAR/CELLULAR ACTION OF CRANIN IN NEURAL CELLS

神经细胞中颅蛋白的分子/细胞作用

基本信息

批准号：
2265776
负责人：
NEIL R SMALHEISER
金额：
$ 9.31万
依托单位：
UNIVERSITY OF CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
1988
资助国家：
美国
起止时间：
1988-12-01 至 1994-11-30
项目状态：
已结题

项目摘要

the extracullular matrix protein laminin is extremely potent in stimulating and guiding the growth of axons. Moreover, it is expressed in the developing and regenerating brain, in parallel with active axonal outgrowth. The effects of laminin may be clinically important in promoting recovery following brain insults, because exogenous grafts containing laminin coated surfacts can permit regeneration of axons to occur within the central nervous system of adult mammals. Neural cells interact with laminin via cell surface molecules. The first step in understanding how laminin's neurite printing effects can be regulated or facilitated is to learn the identities and roles of the laminin-binding proteins found on neural cell surfaces. This proposal tests the hypothesis that a newly discovered laminin-binding protein, cranin, is critical in mediating some of laminin's actions on neural cells. Antibodies will bs raised against cranin that block its binding to laminin. Neural cells will be exposed to these antibodies to test whether their behavioral responses to laminin will be altered. These or other anti-cranin antibodies will also be alloyed to describe the pattern of cranin's expression in developing and regenerating brain, and to examine possible structural modifications of cranin that occur as a function of developmental stags, types of tissue, or under different physiologic conditions. the binding of cranin to laminin will also be examined, for example to test whether cranin binds to a domain of laminin with known neurite promoting activity or other#r bioactivities. Thus. a broad body of both direct and correlative evidence will be adduced to suggest the nature of cranin's roles in neural cells. The molecular cloning of cranin will be completed. This will allow its amino acid sequence and other detailed structural features to be deduce. Furthermore, cranin deficient cells will be transfected with cranin cDNA and assayed for new responsiveness to laminin. This will provides a conclusive test of cranin's proposed role as a laminin 'receptor'. Cranin may be critical in regulating the ability of axons to extend or regenerate under certain conditions, hence it is important to understand cranin's structure and function. Information gained via cloning may lead to news means of controlling the expression of cranin in brain, and thus promote optimal axonal growth both in the fetus and in the adult human following insults.

胞外基质蛋白层粘连蛋白是非常有效的

项目成果

期刊论文数量（17）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Acute neurite retraction triggered by lysophosphatidic acid: timing of the inhibitory effects of genistein.

溶血磷脂酸引发的急性神经突回缩：金雀异黄素抑制作用的时机。

DOI：
10.1016/0006-8993(94)91304-8
发表时间：
1994
期刊：
Brain research
影响因子：
2.9
作者：
Smalheiser,NR;Ali,JY
通讯作者：
Ali,JY

Characterization of a novel set of membrane antigens associated with axonal growth. II. Expression in the chick central nervous system.

与轴突生长相关的一组新型膜抗原的表征。

DOI：
10.1016/0165-3806(92)90163-q
发表时间：
1992
期刊：
Brain research. Developmental brain research
影响因子：
0
作者：
Smalheiser,NR;Collins,BJ
通讯作者：
Collins,BJ

Expression of Egr-1 in the brain of sleep deprived rats.

睡眠剥夺大鼠大脑中 Egr-1 的表达。

DOI：
10.1016/0169-328x(93)90015-h
发表时间：
1993
期刊：
Brain research. Molecular brain research
影响因子：
0
作者：
Landis,CA;Collins,BJ;Cribbs,LL;Sukhatme,VP;Bergmann,BM;Rechtschaffen,A;Smalheiser,NR
通讯作者：
Smalheiser,NR

Acute neurite retraction elicited by diverse agents is prevented by genistein, a tyrosine kinase inhibitor.

金雀异黄素（一种酪氨酸激酶抑制剂）可以防止由多种药物引起的急性神经突回缩。