NEONATAL NUTRITION AND RESONANCE IONIZATION SPECTROSCOPY

对新生儿微量元素营养缺乏了解 要求，特别是早产儿，其关键的最后 三个月必需微量元素的积累突然停止， 出生 这项建议的目的是发展科学， 技术“桥梁”需要证明直接的可行性，或 几乎直接的，用共振电离质谱法测定 （RIMS）的亚纳克量的钼，铜，和V在小于或等于 0.1 cm-3的早产儿血样。 具体而言，目标 （1）研究和应用共振电离方案， Mo、Cu、V的元素选择性电离;（2）发展同位素 样品稀释分析方法 （3）论证了测量亚纳克的可行性 Mo、Cu和V的量;（4）演示如何集成和 上述组件的应用可以导致显著的进步， 新生儿营养的概念，以及这种新的超微量分析 该仪器可扩展并广泛应用于元素和同位素分析。 较长期的目标（第二阶段）是：（1）设计和 构建一个具有成本效益的商业原型RIMS系统， 结合了结构和使用的简单性， 和同位素测量的灵活性;（2）演示应用 从第一阶段到解决具体问题的知识和洞察力， 新生儿营养

A paucity of knowledge exists about neonatal trace element nutrition requirements, especially for premature infants, whose critical final trimester accumulation of essential trace elements is abruptly ceased at birth. The objective of this proposal is to develop the science and technology "bridge" required to demonstrate the feasibility of direct, or nearly direct, determination by resonance ionization mass spectrometry (RIMS) of sub-nanogram quantities of Mo, Cu, and V in less than or equal to 0.1 cm-3 blood samples from premature infants. Specifically, the aims are: (1) To investigate and apply resonance ionization schemes for the elementally selective ionization of Mo, Cu, and V; (2) To develop isotope dilution analysis methodology for samples with minimal chemical preparation; (3) Demonstrate the feasibility of measurement of sub-nanogram quantities of Mo, Cu, and V; (4) Demonstrate how the integration and application of the above components can lead to the significant advancement of neonatal nutrition and how the concept of this new ultratrace analytical tool can be expanded and applied broadly to elements and isotope analysis. The longer term (Phase II) objectives would be: (1) To design and construct a cost effective commercial prototype RIMS system that incorporates simplicity of construction and use and a maximum of elemental and isotopic measurement flexibility; (2) To demonstrate the application of knowledge and insight from Phase I to the solution of specific problems in neonatal nutrition.