FAST DESORPTION AND MASS ANALYSIS OF LARGE MOLECULES

大分子的快速解吸和质量分析

• 批准号: 3505111
• 负责人: LARRY J MOORE
• 金额: $ 5万
• 依托单位: ATOM SCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-01-05 至 1987-07-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3505111
• 关键词: biotechnology; clinical biomedical equipment; ion cyclotron resonance spectrometry; ionization; macromolecule; mass spectrometry; molecular weight

Emerging health and medical research problems are placing stringent requirements on the ability to characterize labile and high molecular weight organic compounds. In biotechnology, for example, intermediate synthesis products must be identified. Recent ion bombardment desorption models suggest that highest molecular weight compounds would desorb most efficiently through rapid multiple excitations near the molecular attachment site. Resonance ionization spectroscopy has the potential to deliver spatially constrained primary ion bundles on target in the picosecond range. The successive ion arrival time intervals for this source are commensurate with subpicosecond times theoretically predicted for molecular motion and energy transfer to the attachment site. The specific aims of this proposal are: 1) modify a thermal atomization source and ion extraction lens for a primary resonance ionization bombardment source; 2) modify an existing time-of-flight mass spectrometer for higher resolution; 3) evaluate the temporal width of the primary ion pulse; 4) investigate the feasibility of efficient desorption of very high molecular weight species. The potential multiple excitation efficiency and temporal resolution of the proposed source would simultaneously provide the bases for desorption of very high molecules and for an ultra-high resolution time-of-flight mass spectrometer.

新出现的健康和医学研究问题 对表征不稳定和 高分子量有机化合物。 在生物技术领域， 例如，必须鉴定中间合成产物。 最近的离子轰击解吸模型表明， 分子量化合物将最有效地解吸 通过在分子附着点附近的快速多重激发 绝佳的价钱 共振电离光谱法有可能 将空间受限的初级离子束递送到靶上， 皮秒范围 连续离子到达时间间隔 该源与亚皮秒时间相当 分子运动和能量传递的理论预测 到附着点。 这项建议的具体目标是：1） 改进热原子化源和离子提取透镜， 主共振电离轰击源; 2）修改 现有的飞行时间质谱仪用于更高的分辨率; 3） 评估初级离子脉冲的时间宽度; 4） 研究高效解吸的可行性， 分子量种类 潜在的多重激励 拟议震源的效率和时间分辨率将 同时提供了基础解吸非常高的 分子和超高分辨率的飞行时间质量 光谱仪