NEONATAL NUTRITION & RESONANCE IONIZATION

痕量和超痕量元素测定的可行性 使用共振电离对微升体积的血清 光谱学（RIS）已在第一阶段建立。 的 开发仪器、方法和应用 有可能进一步加深我们对 早产儿体内的微量金属 微量元素积累 发生在怀孕的最后三个月， 而早产会在关键时刻打断这一过程。 对微量元素的营养需求知之甚少 在早产儿中，或关于微量金属与 观察到的功能障碍，因为可用的血液量较少 0.1毫升。 本提案的具体目标是：1设计， 制造和评估商业原型仪器， RIS用于测定超微量金属; 2）完成 低出生体重儿（1000-2000克）产前微量金属研究 足月婴儿; 3）制定分析服务的方法 使用超微型技术和RIS。 开发的技术 将提供一个商业化的原型RIS仪器， 微量元素和同位素测定，以及 服务分析，为妇科学和医学界。 痕量金属的基线研究将首先进行 早产儿使用小于0.1 ml的样本量。

The feasibility of trace and ultra-trace element determinations on microliter volumes of blood serum using resonance ionization spectroscopy (RIS) has been established in Phase I. The development of instrumentation, methodology and applications has the potential to further our understanding of the role of trace metals in premature infants. Trace element accumulation in the human fetus occurs during the last trimester of pregnancy, and premature birth interrupts this process at a critical time. Very little is known about trace element nutrition requirements in premature infants, or about the relationship of trace metals to observed dysfunctions, since the amount of blood available is less than 0.1 ml. The specific aims of this proposal are to: 1 design, fabricate and evaluate a commercial prototype instrument on RIS to determine ultramicro quantities of metals; 2) complete a study of trace metals in low birth weight (1000-2000 grams) pre- term infants; 3) develop a methodology for analytical services using ultra-micro techniques and RIS. The technology developed will provide a commercial prototype RIS instrument for ultra- trace element and isotope determinations, and the basis for service analyses for the neonatology and medical communities. The baseline study of trace metals will be the first performed with premature infants using sample sizes less than 0.1 ml.