A COST EFFECTIVE CCD DNA SEQUENCE READER

本项目将开发最先进的装药的应用 耦合器件（CCD）图像探测器的自动化问题 DNA测序凝胶分析。 该项目的成功将导致 能够阅读、解释和翻译的仪器的商业化 直接从DNA的放射自显影图存储DNA序列信息 测序凝胶。 这样一个工具将加强基础和医疗 研究社区。 第一阶段将提供用于分析的实验数据 本应用中使用的CCD数字化仪所需的参数。 提出了仪器的物理设计与 将彻底开发图像分析软件 研究了 分析算法和初始软件将 开发 这些数据将作为全面开发的基础 DNA序列凝胶数字化仪的全面开发 第二阶段的相关分析软件。

This project will develop the application of state-of-the-art charge coupled device (CCD) image detectors to the problem of the automatic analysis of DNA sequencing gels. Success of this project will result in commercialization of an instrument capable of reading, interpreting and storing DNA sequence information directly from an autoradiogram of a DNA sequencing gel. Such an instrument will strengthen the basic and medical research communities. Phase I will provide experimental data for analysis of the parameters required of a CCD digitizer used in this application. The problems of integrating the physical design of the instrument with the development of software for the image analysis will be thoroughly investigated. Algorithms of analysis and initial software will be developed. These data will serve as a basis for the full scale development of a DNA sequence gel digitizer including the comprehensive development of related analysis software in Phase II.