NOVEL SUPPORTS FOR CARBOHYDRATE IMMOBILIZATION

碳水化合物固定化的新支持

• 批准号: 3488962
• 负责人: SHAWN G DUNKIRK
• 金额: $ 5万
• 依托单位: SURMODICS, INC.
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-02-01 至 1989-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3488962
• 关键词: affinity labeling; bacterial antigens; biotechnology; carbohydrates; chemical synthesis; covalent bond; crosslink; enzyme linked immunosorbent assay; macromolecule; photochemistry; polyethylenes; polysaccharides; polystyrenes; polyvinyls; radiotracer; reagent /indicator; surface property

The immobilization of biologically active molecules on support matrices has many applications in biotechnology. Passive absorption of biomolecules (both hydrophobic and ionic) to polymeric matrices is the least complicated method of immobilization; however, many biochemicals do not absorb well to plastic polymer surfaces. In particular, carbohydrates are often difficult to absorb with a sufficient degrees of tenacity. Facile, covalent immobilization of carbohydrate moieties would have application in the surface-modification of implantable devices and make possible the detection of carbohydrate antigens in enzyme linked immunosorbent assay (ELISA) procedures. We propose to use Bio-Metric System's proprietary technologies to demonstrate photochemical immobilization of polysaccharides to intractable polystyrene surfaces. The technology expected to result from this project will lead to the development of derivatized support materials for covalent linkage of many carbohydrates by procedures which are less cumbersome than other covalent linkage protocols.

生物活性分子在载体上的固定 基质在生物技术中有许多应用。 被动 生物分子（疏水性和离子性）的吸收， 聚合物基质是最简单的方法， 固定化;然而，许多生物化学品不能很好地吸收， 塑料聚合物表面。 尤其是碳水化合物， 难以以足够程度的韧性吸收。 很容易， 碳水化合物部分的共价固定将具有 在可植入装置的表面改性中的应用， 使酶中糖抗原的检测成为可能 连接的免疫吸附测定（ELISA）程序。 我们建议使用生物计量系统的专有技术， 证明了多糖的光化学固定， 难处理的聚苯乙烯表面。 该技术预计将 该项目的结果将导致开发 用于共价键合许多 碳水化合物的程序，这是不那么繁琐比其他 共价连接方案。