IMMOBILIZATION OF ANTIMICROBIAL PEPTIDES

抗菌肽的固定化

• 批准号: 3489004
• 负责人: SHAWN G DUNKIRK
• 金额: $ 5万
• 依托单位: SURMODICS, INC.
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-06-01 至 1990-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3489004
• 关键词: Staphylococcus; antiinfective agents; autoradiography; biomaterial development /preparation; covalent bond; enzyme linked immunosorbent assay; iatrogenic disease; microorganism culture; peptides; polystyrenes; polyvinyls; prosthesis; radiotracer; silicone rubber; surface property; synthetic peptide

Prosthetic devices are routinely implanted in patients, however an estimated 850,000 cases of implantation infections occur in the U.S. each year. The infectious organisms are most likely introduced at the time of surgery. Currently, infections resulting from implantation are treated as they occur, however prevention is the most promising approach to decreasing device-associated septicemia. One likely method of impeding the attachment and subsequent growth of pathogens is to coast implantable materials with antimicrobials. In this Phase I effort, we propose to covalently couple wide-spectrum antimicrobials (magainins) to model inert surfaces. The Magainins are well studied peptides which act by lysing pathogens at the cell wall and can therefore be active even when immobilized; i.e., they do not need to be internalized. The anticipated results from this Phase I project will demonstrate the feasibility of covalently coupling magainins to produce anti-microbial surfaces. During Phase II, we will optimize coupling chemistries and expand the testing of this technology to other model support matrices and implantable devices.

假体装置常规植入患者体内，然而， 据估计，美国每年发生85万例植入感染， 年 传染性微生物最有可能是在 手术 目前，植入引起的感染被视为 然而，预防是最有希望的方法， 设备相关败血症。 一种可能的阻止连接的方法 病原体的生长会使可植入材料 抗菌剂 在第一阶段的努力中，我们建议共价偶联广谱 抗菌剂（magainins）来模拟惰性表面。 Magainin一家很好 研究了通过裂解细胞壁病原体起作用的肽， 因此即使在固定时也是有活性的;即，它们不需要 内化。 第一阶段项目的预期成果将 证明共价偶联爪蟾抗菌肽以产生 抗菌表面。 在第二阶段，我们将优化耦合 并将该技术的测试扩展到其他型号 支持基质和可植入装置。