LEAD IN SALIVA

唾液中的铅

• 批准号: 3500083
• 负责人: WILLIFRIED SCHRAMM
• 金额: $ 5万
• 依托单位: PACIFIC BIOMETRICS, INC.
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 1993-03-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3500083
• 关键词: atomic absorption spectrometry; blood tests; diagnosis design /evaluation; lead; mass screening; method development; noninvasive diagnosis; saliva

The objective of this project is to investigate the potential of saliva as a biological medium that can be non-invasively obtained for the screening of elevated lead levels in humans. The goals of the feasibility study are threefold: (a) to select a convenient method of specimen collection; (b) to adapt an analytical method to measure the concentrations of lead expected in the specimen; and (c) to determine the correlation of lead in blood and saliva from simultaneously collected specimens. Saliva will be collected either as an ultrafiltrate collected in the mouth with an osmotic device, or by means of a special swab, or as whole saliva either expelled directly or collected with a pipette. The concentration of lead in saliva will be measured by graphite furnace atomic absorption spectroscopy (AAS) with palladium as a modifier. Lead concentrations in saliva are normally lower than concentrations in blood. Therefore, the sensitivity of the AAS method will be enhanced 50 to 100-fold to make the reliable measurement of lead in saliva possible. Finally, matched samples of blood and saliva will be collected from individuals exposed to a different extent to lead and the concentrations will be correlated. These investigations will provide the groundwork for a comprehensive study, performed in Phase II, that results in the recommendation of threshold levels for lead in saliva as specimen.

该项目的目的是研究唾液作为 可以非侵入性地获得用于筛选的生物介质， 人体铅含量升高的证据 可行性研究的目标是 三方面：（a）选择方便的标本采集方法;（B） 采用分析方法测量预期的铅浓度 （c）确定血液中铅含量的相关性， 同时采集的唾液样本。 唾液将被收集 或者作为用渗透装置收集在口中的超滤液， 或者通过特殊的拭子，或者作为整个唾液直接排出 或用移液管收集。 唾液中铅的浓度 通过石墨炉原子吸收光谱法（AAS）测量， 钯作为改性剂。 唾液中的铅浓度通常较低 血液中的浓度。 因此，AAS方法的灵敏度 将增强50至100倍，使铅的可靠测量， 可能是唾液 最后，匹配的血液和唾液样本将 从暴露于不同程度铅的个体中收集， 浓度是相关的。 这些调查将提供 为第二阶段进行的全面研究奠定基础， 建议唾液样本中铅的阈值水平。