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SOLID PHASE ASSAY FOR SALIVA

唾液固相测定

基本信息

批准号：
3508118
负责人：
WILLIFRIED SCHRAMM
金额：
$ 11.03万
依托单位：
PACIFIC BIOMETRICS, INC.
依托单位国家：
美国
项目类别：
财政年份：
1985
资助国家：
美国
起止时间：
1985-07-01 至 1990-02-14
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3508118
关键词：
cell fusion colorimetry drug abuse drug screening /evaluation high performance liquid chromatography hybridomas immunologic techniques monoclonal antibody saliva solid state steroids

项目摘要

This research project is designed to lead to the development of a system for the direct, rapid, reliable and accurate immunoassay of low molecular weight compounds in human saliva. The envisioned system is one that will be easy to use, will not require extraction or chromatography, and will give colorimetric endpoints that can be either qualitative and field- adaptable or, with suitable instrumentation, quantitative. The system will be built upon a novel device that serves to collect and process saliva automatically and unobtrusively yielding in a few minutes several mL's of clear, sterile, protein free filtrate containing compounds of low molecular weight. These compounds are often in equilibrium with the free fraction of the same substances in serum. The feasibility of this saliva collection device and a solid-phase assay system for progesterone were demonstrated during Phase I research. During Phase II it is proposed to extend these investigations to additional compounds, to combine the technologies, and to determine optimal conditions needed to demonstrate commercial feasibility of the approach. In particular during Phase II it is proposed to develop conditions for construction of the saliva collection system that will be acceptable to users and optimal for saliva collection, analyte processing, and colorimetric immunoassay performance; prepare derivatives and analyte- enzyme conjugates suitable for immunization and colorimetric immunoassay; develop monoclonal antibodies suitable for colorimetric immunoassay of the selected compounds; and develop and validate several colorimetric solid phase immunoassays for these compounds in human saliva. Immunoassay development during Phase II will focus on the steroids testosterone, progesterone, cortisol and estriol; the prostanoid thromboxane; and a major metabolite of cocaine, benzoylecgonine.

本研究项目旨在引导一个系统的开发用于直接、快速、可靠、准确的低分子免疫分析人类唾液中的重量化合物。设想的系统将是一个易于使用，不需要提取或层析，并将提供色度端点，既可以是定性的，也可以是现场的- 适应性强，或在适当的工具下，量化的。系统将建立在一种用于收集和处理唾液的新型设备上在几分钟内自动且不引人注目地产生几毫升的透明、无菌、无蛋白的滤液，含有低分子化合物重量。这些化合物通常与游离态的在血清中也有同样的物质。收集唾液的可行性介绍了一种测定黄体酮的装置和固相检测系统在第一阶段研究期间。在第二阶段期间，建议延长这些对其他化合物的研究，对技术的结合，以及对确定证明商业可行性所需的最佳条件这种方法的重要性。特别是在第二阶段期间，建议制定唾液收集系统的建设条件将是用户可以接受，最适合唾液收集、分析物处理，和比色免疫分析性能；准备衍生物和分析物- 适用于免疫和比色免疫分析的酶结合物；建立适合于比色免疫分析的单抗选定的化合物；并开发和验证几种比色固体人唾液中这些化合物的时相免疫分析。免疫分析第二阶段的开发将专注于类固醇和睾丸素，黄体酮、皮质醇和雌三醇；前列腺素类血栓烷；可卡因的代谢物，苯甲酰ecGonine。