IMMUNOSENSOR FOR DRUG MONITORING

用于药物监测的免疫传感器

• 批准号: 3507119
• 负责人: WILLIFRIED SCHRAMM
• 金额: $ 24.13万
• 依托单位: PACIFIC BIOMETRICS, INC.
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 1993-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3507119
• 关键词: biomedical equipment development; biosensor device; blood glucose; body fluids; clinical biomedical equipment; cocaine; diagnosis design /evaluation; drug abuse; drug metabolism; drug screening /evaluation; electrochemistry; human tissue; immunochemistry; immunoconjugates; immunologic assay /test; immunologic techniques; rapid diagnosis

For the efficient screening of drugs of abuse, simple but unambiguous analytical methods are required which can be performed on-site. This project is designed to develop an immunosensor for the detection of drugs of abuse. While our project is focused on the detection of benzoylecgonine, the major urinary metabolite of cocaine, immomunosensor technology can be readily adapted to other substances for which antibodies are available, including therapeutic drugs and other physiologically active compounds. Operation of the immunosensor is a single-step procedure which does not require the usual steps associated with immonoassays such as pipetting, adding of reagents, manual separation and mixing; the disposable sensor need only be dipped into the biological medium (urine, blood, saliva). The signal generated will be monitored with existing technology that has been developed for the measurement of blood glucose. Once the sensor is dipped in the biological medium, the assay will be complete in a minimum of 3 to 10 minutes, without the need to stop the reaction when it is complete.

对于滥用药物的有效筛查，简单但明确 需要可在现场进行的分析方法。这 项目旨在开发用于检测药物的免疫传感器 虐待虽然我们的项目集中在检测苯甲酰芽子碱， 可卡因的主要尿代谢物，免疫传感器技术可以 容易适应于其他可获得抗体的物质， 包括治疗药物和其它生理活性化合物。 免疫传感器的操作是一个单步程序， 需要与免疫测定相关的常规步骤如移液， 添加试剂，手动分离和混合;一次性传感器 只需要浸入生物介质（尿液、血液、唾液）。的 所产生的信号将使用现有技术进行监测， 用于测量血糖。一旦传感器被浸入 在生物培养基中，测定将在至少3至 10分钟，反应完成后无需停止。