RAPID CYTOMETRIC ASSAY FOR LYMPHOCYTE ACTIVATION

淋巴细胞活化的快速细胞测定法有很多潜力 临床应用，包括细胞免疫功能障碍的评估 在HIV感染和癌症中，评估自身免疫的活性 移植受者的疾病过程、组织分型和监测 以寻找移植排斥的早期迹象。为了……的发展 可重复的、标准化的方法，可在当前 临床流式细胞仪的产生，以及较新的细胞仪 仪器即将投入使用，将使用多参数流式细胞仪 分析和关联胞浆pH、荧光素的变化 人的荧光偏振与活化抗原的表达 外周T淋巴细胞在激活后24小时内 多克隆有丝分裂原植物血凝素或由同种异体抗原混合 淋巴细胞反应。拟议中的研究很可能会产生 基于pH测量和激活抗原检测的可用的检测。

Rapid cytometric assays for lymphocyte activation have many potential clinical applications, including evaluation of cellular immune dysfunction in HIV infection and cancer, assessment of the activity of autoimmune disease processes, tissue typing, and monitoring of transplant recipients for early signs of graft rejection. With a view toward the development of reproducible, standardized methods which can be implemented on the current generation of clinical flow cytometers, as well as on newer cytometric apparatus now coming into use, multiparameter flow cytometry will be used to analyze and correlate changes in cytoplasmic pH, fluorescein fluorescence polarization, and activation antigen expression of human peripheral T-lymphocytes during the first 24 hours following activation by the polyclonal mitogen phytohemagglutinin or by alloantigen in the mixed lymphocyte reaction. It is likely that the proposed studies will yield usable assays based on pH measurement and activation antigen detection.