RAPID CYTOMETRIC ASSAY FOR LYMPHOCYTE ACTIVATION

淋巴细胞活化的快速细胞计数检测具有许多潜力 临床应用，包括细胞免疫功能障碍的评价 在HIV感染和癌症中，评估自身免疫性疾病的严重程度， 疾病过程、组织分型和移植受者监测 移植排斥反应的早期迹象 为了发展 可重复的，标准化的方法，可以在当前的 临床流式细胞仪的一代，多参数流式细胞术将 用于分析和关联RNA含量、激活 抗原表达和膜示踪染料荧光 外周血T淋巴细胞在活化后的前24小时内 多克隆有丝分裂原，同种异体抗原，或不匹配的细胞，在试验中， 比较这些细胞活化的细胞计数指标的功效 氚标记胸苷掺入。 很可能这些研究 将使用以下方法验证基于RNA含量增加检测的测定 派洛宁Y和/或CD 98活化抗原的表达。 拟议的商业应用：淋巴细胞活化的快速检测 在诊断和监测中具有广泛的潜在应用范围， 免疫系统紊乱

Rapid cytometric assays for lymphocyte activation have many potential clinical applications, including evaluation of cellular immune dysfunction in HIV infection and cancer, assessment of the severity of autoimmune disease processes, tissue typing, and monitoring of transplant recipients for early signs of graft rejection. With a view toward development of reproducible, standardized methods which can be implemented on the current generation of clinical flow cytometers, multiparameter flow cytometry will be used to analyze and correlate changes in RNA content, activation antigen expression, and membrane tracking dye fluorescence in human peripheral T-lymphocytes during the first 24 hours following activation by polyclonal mitogens, alloantigen, or mismatched cells, in a trial of the efficacy of these cytometric indicators of cell activation in comparison with tritiated thymidine incorporation. It is likely that these studies will validate an assay based on detection of RNA content increases using pyronin Y and/or expression of the CD98 activation antigen. PROPOSED COMMERCIAL APPLICATION: Rapid tests for lymphocyte activation have a wide potential range of application in diagnosis and monitoring of immune system disorders.