METABOLIC IMAGING OF LIVERS AWAITING TRANSPLANT

本研究的长期目标是开发无创光学 用于评估冷损伤的功能完整性的仪器和方法， 在移植前储存肝脏。 这项研究是基于工作 假设在低温储存的肝脏中， 氧化代谢可以用非侵入性方法检测， 分光光度法 荧光检测NADH和可见光 细胞色素氧化还原变化的光反射检测将用于 在常氧条件下检查低温灌注和冷藏的大鼠肝脏， 缺血性和非偶联性病症。 I期研究将证明 工作假设的可行性。 设备将被组装， 成像肝脏NADH荧光和测量细胞色素反射光谱 从低温灌注和冷藏的大鼠肝脏。 相关性 将产生具有肝功能和功能障碍的光学变化。 的 开发的方法将评估其灵敏度， 区分线粒体代谢受损的区域 正常的组织。 在成功演示工作后， 假设，第二阶段将使这些概念适应成像的发展 方法和在肝移植的临床环境中的测试 位于华盛顿西雅图港景医疗中心的中心。

The long term goal of this research is to develop noninvasive optical instruments and methods for assessing the functional integrity of cold- stored livers prior to transplant. This research is based on the working hypothesis that in hypothermically-stored livers, areas of dysfunctional oxidative metabolism can be detected noninvasively using spectrophotometric methods. fluorescence detection of NADH and visible light reflectance detection of cytochrome redox changes will be used to examine hypothermic perfused and cold-stored rat livers under normoxic, ischemic and uncoupled conditions. The Phase l studies will demonstrate the feasibility of the working hypothesis. Equipment will be assembled to image hepatic NADH fluorescence and measure cytochrome reflectance spectra from both hypothermic perfused and cold-stored rat livers. Correlation of optical changes with hepatic function and dysfunction will be made. The methods developed will be evaluated for their sensitivity for distinguishing areas of impaired mitochondrial metabolism on a background of normal tissue. Upon successful demonstration of the working hypothesis, Phase II will adapt these concepts to development of imaging methods and to testing in a clinical environment at the liver transplant center at Harborview Medical Center, Seattle, Washington.