FLUORESCENCE IMAGING OF REGIONAL FLOW IN HEART

心脏区域血流的荧光成像

• 批准号: 6183450
• 负责人: Jeffrey J Kelly
• 金额: $ 34.6万
• 依托单位: BARLOW SCIENTIFIC, INC.
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 2001-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6183450
• 关键词: bioimaging /biomedical imaging; biomedical equipment development; cardiovascular imaging /visualization; dogs; fluorescein angiography; fluorescent dye /probe; laboratory rat; method development; microcapsule; microcirculation; swine

This research involves the development of fully-automated instrumentation procedures to evaluate microregional circulation in tissues. Phase I results have demonstrated that cell-permeant fluorescent dyes or "molecular microspheres" can be spatially located in sequential fluorescence images recorded from frozen tissue blocks during organ serial sectioning. Statistical limitations imposed on current fluorescent and radioactive microsphere methods are removed by the use of molecule-sized microspheres, expanding greatly the details of organ-flow mapping. During Phase I, several dyes have been identified as having excellent retention in heart vascular systems and their regional deposition correlates strongly with simultaneously injected 15-micron diameter fluorescent microspheres. Both the validity of the molecular microsphere approach and the feasibility of the imaging methodology have been demonstrated. Phase II will concentrate on adapting and optimizing the imaging system for microregional flow imaging and will involve a rigorous validation of the technique through collaboration with the Fluorescent Microsphere Resource Center at the University of Washington. This project will provide researchers with instrumentation for rapid, automatic evaluation of microregional flow in tissue volumes less than 1/1000 the size of those possible with current techniques, while using procedures similar to the standard procedures involved in radioactive and fluorescent microsphere flow determinations. PROPOSED COMMERCIAL APPLICATIONS: Instrumentation and methods to rapidly and automatically evaluate molecular microsphere distributions in tissue will find applications in laboratories engaged in physiology and circulation research. The development of molecular microsphere methodology in the proposal will significantly enhance the commercial market for the Barlow Scientific microsphere imaging system in such laboratories.

这项研究涉及全自动仪器程序的开发，以评估组织中的微区域循环。第一阶段的结果表明，细胞渗透荧光染料或“分子微球”可以在空间上位于连续的荧光图像记录从冷冻组织块在器官连续切片。目前的荧光和放射性微球的方法施加的统计限制被删除的分子大小的微球的使用，大大扩展了器官流映射的细节。在阶段I期间，几种染料已被鉴定为在心脏血管系统中具有优异的保留，并且它们的区域沉积与同时注射的15微米直径的荧光微球强烈相关。分子微球方法的有效性和成像方法的可行性已被证明。第二阶段将集中调整和优化微区域流动成像的成像系统，并将通过与华盛顿大学荧光微球资源中心的合作，对该技术进行严格的验证。该项目将为研究人员提供快速、自动评估组织体积中微区域流量的仪器，该体积小于当前技术可能尺寸的1/1000，同时使用与放射性和荧光微球流量测定中涉及的标准程序类似的程序。拟议的商业应用：快速自动评估组织中分子微球分布的仪器和方法将在从事生理学和循环研究的实验室中得到应用。该提案中分子微球方法学的开发将显着增强Barlow Scientific微球成像系统在此类实验室中的商业市场。