FLUORESCENCE IMAGING OF REGIONAL FLOW IN HEART
心脏区域血流的荧光成像
基本信息
- 批准号:2423728
- 负责人:
- 金额:$ 10万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1997
- 资助国家:美国
- 起止时间:1997-09-30 至 1998-03-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
This research will develop instrumentation for a fully-automated
approach to the evaluation of microregional circulation. This
research is based on the working hypothesis that cell permeant
fluorescent dyes or "molecular microspheres" can be spatially
located by sequential fluorescence imaging of frozen tissue blocks
during organ serial sectioning.. The project will develop procedures
and adapt instrumentation to evaluate regional flow in an entire
organ. During Phase I, feasibility of the technical methodology will
be demonstrated through examination of dye optical and extraction
properties as well as dye washout and distribution. The validity of
the molecular microsphere method will be demonstrated by comparing
dye distributions with distributions of 15 microM diameter microspheres
simultaneously injected into coronary circulation. Phase I studies
will demonstrate feasibility of the working hypothesis and provide
design parameters for methods to work with larger hearts and for
working with multiple dyes simultaneously. This project will provide
researchers with the instrumentation to rapidly and automatically
evaluate microregional flow in tissue using procedures similar to
the standard procedures involved in radioactive and fluorescent
microsphere flow determinations.
PROPOSED COMMERCIAL APPLICATIONS Instrumentation and methods to
rapidly and automatically evaluate molecular microsphere
distributions in tissue will find applications in laboratories
engaged in physiology and circulation research. The development of
molecular microsphere methodology in the proposal will significantly
enhance the commercial market for the Barlow Scientific microsphere
imaging system in such laboratories.
本研究将开发全自动化的仪器
项目成果
期刊论文数量(0)
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Jeffrey J Kelly其他文献
Jeffrey J Kelly的其他文献
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