Antibody Imaging for Regional Flow Measurements

描述（由申请人提供）： 这项研究涉及到一种方法的发展，量化区域流动组织使用自动化仪器程序。第一阶段的结果表明，荧光抗体，特异性抗原在毛细血管内皮细胞，可以在空间上定位在连续的荧光图像记录从冷冻组织块在器官连续切片。荧光和放射性微球的方法施加的统计限制被删除的使用分子作为流动标记（分子微球），大大扩展了器官流图的细节。在第一阶段，荧光抗体被鉴定为在心脏血管系统和区域沉积中具有优异的保留，这与同时注射的15微米直径的荧光素微球强烈相关。分子微球方法的有效性和成像方法的可行性已被证明。第二阶段将集中在心脏的体内研究，分析程序的开发和肺和脑的验证研究将由合作者进行。还将开发在同一器官中进行多次流量测量的方法。该项目将为研究人员提供方法和仪器，以测量比现有技术小得多的组织体积中的区域流量。

DESCRIPTION (provided by applicant): This research involves the development of a method to quantify regional flow in tissues using automated instrumental procedures. Phase I results have demonstrated that fluorescent antibodies, specific to antigens in capillary endothelium, can be spatially located in sequential fluorescence images recorded from frozen tissue blocks during organ serial sectioning. Statistical limitations imposed on fluorescent and radioactive microsphere methods are removed by the use of molecules as flow markers (molecular microspheres), greatly expanding the details of organ-flow maps. During Phase I, fluorescent antibodies were identified that have excellent retention in heart vascular systems and regional deposition which correlates strongly with simultaneously injected 15 micron-diameter flourescent microspheres. Both the validity of the molecular microsphere approach and the feasibility of the imaging methodology have been demonstrated. Phase II will concentrate on in vivo studies in heart, development of analysis procedures and validation studies in lung and brain to be undertaken by collaborators. Methods will also be developed to conduct multiple flow measurements in the same organ. This project will provide researchers with methods and instrumentation to measure regional flow in tissue volumes much smaller than those possible with current techniques.