IMMUNODIAGNOSTIC FOR MEASURING PROLIFERATION--HUMAN CELL

用于测量增殖的免疫诊断——人体细胞

• 批准号: 3492230
• 负责人: DAVID H BING
• 金额: $ 5万
• 依托单位: CBR LABORATORIES, INC.
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-29 至 1990-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3492230
• 关键词: B lymphocyte; Burkitt's lymphoma; T lymphocyte; cell growth regulation; glycolipids; human tissue; image processing; immunocytochemistry; immunofluorescence technique; laboratory mouse; lymphoma; monoclonal antibody; neoplasm /cancer immunodiagnosis; neoplastic transformation; surface antigens

Evaluation of tissues for the presence of cancer cells on abnormal tissues is an important and crucial step in the diagnosis, disease management and control of cancer. We have generated a mouse monoclonal antibody which detects a glycolipid antigen (PAA) on proliferating T- cells, B-cells and tumor cells in both frozen and paraffin fixed tissues. We hypothesize that level of binding of this antibody to a cell reflects its proliferation potential. We will test this concept in a series of experiments which include in situ comparative immunohistochemical staining of proliferating cells in lymphomas and a variety of solid tumors with anti-PAA monoclonal antibody that is used to stain proliferating cells in frozen sections of normal and cancer tissue. We will use digital image analysis and immunofluorescence staining for the in situ studies. The results of these studies will determine the feasibility of using anti-PAA monoclonal antibody as an immunodiagnostic reagent for detection of proliferating cells in tumors.

评估组织中异常癌细胞的存在 组织是诊断疾病的重要和关键步骤， 癌症的管理和控制。我们已经产生了一个小鼠单克隆 检测增殖T细胞上糖脂抗原（PAA）的抗体， 细胞、B细胞和肿瘤细胞。 我们假设这种抗体与细胞的结合水平反映了 其扩散潜力。我们将在一系列的测试中验证这一概念， 包括原位比较免疫组织化学 淋巴瘤和各种实体瘤中增殖细胞的染色 用抗PAA单克隆抗体染色肿瘤 正常和癌组织的冷冻切片中的增殖细胞。我们 将使用数字图像分析和免疫荧光染色， 现场研究。这些研究的结果将决定 利用抗PAA单克隆抗体作为免疫诊断的可行性 用于检测肿瘤中增殖细胞的试剂。