AFFINITY ADSORBENTS FOR THE REMOVAL OF HIV-I FROM BLOOD

建议开发一种亲和吸附系统，该系统可以 用于从溶液中去除HIV-1抗原和/或病毒。这个 膜将使用一种新的配体，即T淋巴细胞CD4 HIV-1的受体。这一蛋白质最近被克隆并 表达为一种完全溶解的50kd蛋白，可以结合 特别是在与HIV-1的gp120包膜蛋白的溶液中。这个 实验计划将是使用新的化学修饰 固相膜上固定化该蛋白的方法 材料。固定化的受体将被充分鉴定 根据其与纯化的gp120的结合亲和力和特异性 和HIV-1与血液中发现的其他病毒的比较 (例如，Epstein Barr、巨细胞病毒)。我们还将尝试使用 合成肽，其序列基于 HIV包膜蛋白的序列，以在竞争中确定 实验病毒受体的活性中心。这些结果 应为设计HIV-1特异性基因提供实验依据 血液兼容的过滤器或吸附材料，可以是 在采血过程中的适当步骤引入或 生产治疗性血浆衍生品。

It is proposed to develop an affinity adsorbent system which can be used to remove HIV-1 antigens and/or virus from solution. The membrane will use a novel ligand, namely the T lymphocyte CD4 receptor for the HIV-1. This protein has recently been cloned and expressed as a fully soluble 50 kd protein which can combine specifically in solution with the gp120 env protein of HIV-1. The experimental plan will be to use novel chemical modification protocols to immobilize this protein on solid phase membrane materials. The immobilized receptor will be fully characterized according to its binding affinity and specificity for purified gp120 and HIV-1 as compared to other viruses which are found in blood (e.g., Epstein Barr, Cytomegalovirus). We will also attempt to use synthetic peptides, the sequence of which is based on the sequence of the HIV env proteins, to ascertain in competition experiments the active center of the viral receptor. These results should provide the experimental basis for designing HIV-1 specific blood compatible filter or adsorbent materials which could be introduced at appropriate steps in the blood collection process or in the manufacture of therapeutic plasma derivatives.