AFFINITY ADSORBENTS FOR THE REMOVAL OF HIV-I FROM BLOOD

用于去除血液中 HIV-I 的亲和吸附剂

• 批准号: 3359141
• 负责人: DAVID H BING
• 金额: $ 39.72万
• 依托单位: IMMUNE DISEASE INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 1991-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3359141
• 关键词: CD4 molecule; adsorption; blood filtration; glycoproteins; human immunodeficiency virus 1; human tissue; protein structure; synthetic peptide; virus antigen; virus envelope; virus protein; virus receptors

It is proposed to develop an affinity adsorbent system which can be used to remove HIV-1 antigens and/or virus from solution. The membrane will use a novel ligand, namely the T lymphocyte CD4 receptor for the HIV-1. This protein has recently been cloned and expressed as a fully soluble 50 kd protein which can combine specifically in solution with the gp120 env protein of HIV-1. The experimental plan will be to use novel chemical modification protocols to immobilize this protein on solid phase membrane materials. The immobilized receptor will be fully characterized according to its binding affinity and specificity for purified gp120 and HIV-1 as compared to other viruses which are found in blood (e.g., Epstein Barr, Cytomegalovirus). We will also attempt to use synthetic peptides, the sequence of which is based on the sequence of the HIV env proteins, to ascertain in competition experiments the active center of the viral receptor. These results should provide the experimental basis for designing HIV-1 specific blood compatible filter or adsorbent materials which could be introduced at appropriate steps in the blood collection process or in the manufacture of therapeutic plasma derivatives.

提出了开发一种具有良好吸附性能的亲和吸附体系