IMAGE PROCESSING IN PRENATAL ANALYSIS

产前分析中的图像处理

• 批准号: 3500090
• 负责人: DAVID H BING
• 金额: $ 5万
• 依托单位: CBR LABORATORIES, INC.
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-03-01 至 1993-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3500090
• 关键词: blood tests; cell cycle; centromere; child (0-11); cytogenetics; female; fluorescent dye /probe; human subject; image processing; in situ hybridization; karyotype; leukocytes; mathematical model; method development; nucleic acid probes; nucleic acid repetitive sequence; prenatal diagnosis; statistics /biometry; tissue /cell culture; trophoblast; video recording system

For prenatal cytogenetic analysis, cells are now sampled from the amniotic sac, a procedure which carries some risk and some women will not submit to it. There is a desire to find a less invasive and less risky sampling procedure. A number of laboratories are developing procedures to enrich fetal trophoblasts from maternal blood. These procedures are not expected to provide pure populations of fetal cells, so a means of verifying fetal origin for female fetuses is needed. To verify fetal origin, we propose to develop a statistics-based image analysis of metaphase chromosomes labelled with fluorescent alpha satellite DNA probes. We base our strategy on the fact that the number of copies of alpha-satellite DNA repeats is variable in human populations, so an analysis of intensity of the fluorescent "light stars" emanating from each chromosome should allow us to distinguish fetal from maternal cells. This procedure uses the same metaphase spreads prepared for standard prenatal cytogenetics, so standard analysis can be carried out on previously stored images of the fetal-verified cells. A simpler version of these procedures will provide a means for uniquely identifying humans for paternity and forensic purposes.

为了进行产前细胞遗传学分析，细胞现在从 羊膜囊，这是一种有一定风险的手术，一些妇女不会 顺从它。人们渴望找到一种侵袭性更小、风险更小的 抽样程序。一些实验室正在制定程序 从母体血液中富含胎儿滋养层细胞。这些程序是 不希望提供纯的胎儿细胞群体，所以一种手段 需要核实女性胎儿的胎儿来源。 为了验证胎儿的来源，我们建议开发一种基于统计的图像 荧光α标记的中期染色体分析 卫星DNA探测器。我们的战略是基于这样一个事实，即 α-卫星DNA重复序列的拷贝数在人类中是不同的 因此分析了荧光“光星”的强度 从每条染色体发出的信号应该能让我们区分胎儿和 母体细胞。 此过程使用与为标准 产前细胞遗传学，因此可以进行标准分析 之前存储的胎儿验证细胞的图像。更简单的版本 这些程序将提供一种唯一识别人类的方法 出于亲子鉴定和取证的目的。