SIMPLIFIED FROZEN BLOOD METHODOLOGY FOR AUTOLOGOUS BLOOD

自体血的简化冷冻血液方法

• 批准号: 3501606
• 负责人: DAVID H BING
• 金额: $ 5万
• 依托单位: CBR LABORATORIES, INC.
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1990-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3501606
• 关键词: autologous transplantation; blood banks; blood donor; blood transfusion; cell death; cell population study; cryopreservation; erythrocytes; human subject

The overall goal of this project is to determine whether frozen red cells stored at -25oC for periods up to six months will have an in vivo survival equal to that of frozen red cells stored at -65oC for periods up to 10 years and will meet the criteria established by the Food and Drug Administration Office of Biological Research and Review (FDA-OBRR) for the blood product Red Blood Cells Frozen. The specific aim of this project is to determine whether red cells frozen and stored at -25oC have sufficient recovery and in vitro biochemical characteristics to justify their use for transfusion therapy, i.e., at least 80% recovery of red cells following deglycerolization, normal 2,3, DPG levels and at least 6760% of the baseline ATP level. These studies are the first part of a development plan that would provide the technology to expand the capabilities of autologous blood donor programs to include the use of a simplified method for the freezing preservation at -25oC, and recovery of red blood cells that are suitable for transfusion. Such a program could potentially have wide use and accptance in community hosptials, blood banks, in underdeveloped areas where sophisticated freezing and storage equipment is not readily available and in the military establishment where every member of the armed forces is a potential blood donor.

这个项目的总体目标是确定是否冻红 细胞在-25 ℃下储存长达6个月， 存活率等于-65 ℃下储存的冷冻红细胞 最多10年，并将符合食品和药物管理局制定的标准， 药品管理局生物研究和审查办公室（FDA-OBRR） 冷冻红细胞的血液制品其具体目的是 项目是确定红细胞是否在-25 ℃下冷冻和储存 具有足够的回收率和体外生化特性， 证明其用于输血治疗的合理性，即，至少80%回收率 去甘油后红细胞的2，3，DPG水平正常， 至少为基线ATP水平的6760%。这些研究是第一部分 一个发展计划，将提供技术，以扩大 自体献血者计划的能力，包括使用 -25 ℃冷冻保存及回收的简化方法 适合输血的红细胞。这样的程序 可能在社区医院有广泛的用途和接受度， 血库，在欠发达地区， 存储设备不是现成的，在军事 武装部队的每一个成员都是潜在的 献血者