OMEGA-3 POLYUNSATURATED FATTY ACIDS FROM ALGAE

膳食补充剂和营养成分之间存在显著的相关性 二十碳五烯酸(EPA)，降低冠状动脉发病率 心脏病，选择性地杀死某些癌细胞和缓解 类风湿性关节炎的症状。我们提出了一种生物技术 一种生产含油环保局的途径 在受控发酵培养中生长的微生物 替代目前可用的鱼油形式的环境保护局。这个 一期筛选出的微生物生产的石油具有较高的 EPA含量，加热稳定性增强，无“鱼腥味” 与商业上可获得的鱼油来源相比，如 最高环保局。菌种选育与菌种改良策略 本提案中概述的内容应为 利用EPA生产含EPA石油在经济上可行 常规发酵技术。此外， 从这些微生物中生物生产EPA提供了重要的 能够生产用于临床研究的标记EPA的额外好处 在短期内。我们的总体目标是产生一个更好的环境保护局- 含有与之兼容且可集成的油 进入，典型的食品在我们的饮食中是积极的 环保局的属性可以被更多的美国人实现，而不是 服用药物的要求。

There is a significant correlation between dietary supplements of eicosapentaenoic acid (EPA), with reduced incidence of coronary heart disease, selective killing of certain cancer cells and relief of symptoms of rheumatoid arthritis. We propose a biotechnological approach for the production of EPA containing oil from microorganisms grown in controlled, fermentation culture as an alternative to the fish oil form of EPA presently available. The oil produced by the microorganisms selected in Phase I had a higher EPA content, enhanced stability upon heating, and no "fishy" odor when compared to commercially available fish oil sources such as MaxEPA. The strain selection and culture improvement strategies outlined in this proposal should provide the foundation for the economically feasible production of EPA-containing oil using conventional fermentation technologies. Furthermore, the bioproduction of EPA from these microorganisms offers a significant bonus in being able to produce labelled EPA for clinical research in the near term. Our overall goal, is to produce a superior EPA- containing oil which is compatible with, and can be integrated into, typical food products in our diet so that the positive attributes of EPA can be realized by more Americans without the requirement of taking pills.