EICOSAPENTAENOIC ACID FROM HETEROTROPHIC MICROALGAE

描述(改编自申请人的摘要)：总体目标 这个项目是为了生产一种从藻类中提取的富含石油 在omega-3多不饱和脂肪酸中。初步数据显示， 是一种硅藻，它产生一种非膜相关的甘油三酯 EPA含量高，但其他多不饱和脂肪酸含量低， 并且对氧化更稳定。然而，这种有机体是一种强制性的 原生生物。该提案旨在将这种菌株转化为异养菌。 从而可以通过发酵大量生产并提供 一种经济的富含EPA的石油来源。建议的战略是 首先确定该菌株是否运输葡萄糖。如果不是这样， 研究人员将尝试使用葡萄糖转运蛋白基因将其转化为 来自另一种微生物。 如果这一策略不成功，完全转化或突变和 将尝试进行选择。在第二阶段，程序将得到优化 利用研发的菌株为“设计者”的商业化做准备 石油。“

DESCRIPTION (Adapted from the Applicant's Abstract): The overall goal of this project is to manufacture an oil derived from algae which is enriched in omega-3 polyunsaturated fatty acids. Preliminary data have shown there is a diatom which produces a non-membrane associated triglyceride with a high content of EPA but low contents of other polyunsaturated fatty acids, and is more stable to oxidation. However, this organism is an obligate prototroph. The proposal aims to transform this strain into a heterotroph so that it can be produced by fermentation in large quantities and provide an economical source of an oil enriched in EPA. The strategy proposed is to first determine if this strain transports glucose. If it doesn't the investigators will try to transform it using the glucose transporter gene from another microorganism. If this strategy is unsuccessful, total transformation or mutagenesis and selection will be attempted. In phase 2, the procedure will be optimized using the developed strain to prepare for commercialization of a "designer oil."