EICOSAPENTAENOIC ACID FROM HETEROTROPHIC MICROALGAE

来自异养微藻的二十碳五烯酸

• 批准号: 3502121
• 负责人: DAVID J KYLE
• 金额: $ 5万
• 依托单位: MARTEK BIOSCIENCE CORPORATION
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-01 至 1992-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3502121
• 关键词: algae; eicosanoids; fermentation; glucose transport; nutrient requirement; nutrition related tag; omega 3 fatty acid

DESCRIPTION (Adapted from the Applicant's Abstract): The overall goal of this project is to manufacture an oil derived from algae which is enriched in omega-3 polyunsaturated fatty acids. Preliminary data have shown there is a diatom which produces a non-membrane associated triglyceride with a high content of EPA but low contents of other polyunsaturated fatty acids, and is more stable to oxidation. However, this organism is an obligate prototroph. The proposal aims to transform this strain into a heterotroph so that it can be produced by fermentation in large quantities and provide an economical source of an oil enriched in EPA. The strategy proposed is to first determine if this strain transports glucose. If it doesn't the investigators will try to transform it using the glucose transporter gene from another microorganism. If this strategy is unsuccessful, total transformation or mutagenesis and selection will be attempted. In phase 2, the procedure will be optimized using the developed strain to prepare for commercialization of a "designer oil."

描述（改编自申请人的摘要）： 这个项目是生产一种从藻类中提取的油， ω-3多不饱和脂肪酸中的含量 初步数据显示， 是一种硅藻，其产生非膜相关的甘油三酯， EPA含量高而其它多不饱和脂肪酸含量低， 并且对氧化更稳定。 然而，这种生物是一种专性的 原养生物 该提案旨在将这种菌株转化为异养菌 因此它可以通过发酵大量生产， 富含EPA的油的经济来源。 建议的战略是 首先确定这个菌株是否能运输葡萄糖。 如果没有， 研究人员将尝试用葡萄糖转运蛋白基因 另一种微生物。 如果该策略不成功，则进行完全转化或诱变， 将尝试选择。 在第二阶段，将优化程序 利用所开发的菌株为“设计者”的商业化做准备， 油"