ROLE OF THE SEMINAL VESICLES IN FERTILITY IN THE HOUSE MICE

精囊在家鼠生育能力中的作用

• 批准号: 3877323
• 负责人: BETSY PEITZ
• 金额: --
• 依托单位: CALIFORNIA STATE UNIVERSITY LOS ANGELES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3877323
• 关键词: fertility; gene expression; genetic strain; laboratory mouse; semen; seminal vesicles; sperm motility; vagina

While a well developed complex of accessory glands is characteristic of the male reproductive tract of most mammals, including man, the role of these glands in fertility is unclear. There appear to be differences between species in the function of these glands. Seminal vesicle (SV) removal in boars and bulls has little or no effect or fertility, while in rodents, SV removal leads to infertility. Even among the rodents, the cause of infertility following SV removal may vary. In the rat, the vaginal plug appears to be necessary for fertility, but the plug is not necessary in the house mouse. In the house mouse, SV removal induced infertility is correlated with changes in uterine sperm motility and SV fluids fractionated by gel filtration have a direct effect on sperm motility in vitro. This study has three aims. The first is to investigate the effects of SV removal on the transport of sperm in the female reproductive tract. These data will indicate whether the reduction in fertility which follows SV removal is a result of failure of the sperm to reach the site of fertilization at the right time or in sufficient numbers. The second goal is to determine whether different genetic strains of mice respond differently to SV removal and whether the degree of fertility reduction in different strains of mice after SV removal is correlated to alterations in sperm motility. If a group of strains with varying degrees of fertility impairment can be identified it may be possible to identify which aspects of sperm motility are most closely linked to fertility. It may also be possible to use such a group of animals to test parameters for predicting or treating infertility. The third goal is to determine whether SV removal also charges the pattern of uterine sperm lectin binding and to decide whether sperm lectin binding studies could be used to predict fertility. The student participants in this project will learn the techniques for videomicrographic analysis of sperm motility and standard techniques for making stained slides of tissues and cells. They will also be exposed to current ideas in the study of fertility. The results of the study should give us an insight into the natural processes which influence fertility in vivo.

而发达的附腺复合体， 大多数哺乳动物雄性生殖道的特征， 包括人类在内，这些腺体在生育中的作用尚不清楚。 不同的物种在功能上似乎存在差异。 这些腺体 公猪和公牛的精囊（SV）去除 对生育力影响很小或没有影响，而在啮齿动物中，SV清除 导致不孕 即使在啮齿动物中， SV移除后的不孕可能不同。 在大鼠中，阴道 塞子似乎是生育所必需的，但塞子不是 必要的房子老鼠。 室内小鼠，SV清除 诱发不孕症与子宫精子的变化有关 通过凝胶过滤分离的运动和SV流体具有直接的 对体外精子活力的影响。 本研究有三个目的。 的 首先是研究SV去除对转运的影响 女性生殖道中精子的数量 这些数据将 表明SV后生育率的降低是否 去除是由于精子未能到达的网站， 在适当的时间或足够的数量受精。 的 第二个目标是确定不同的遗传菌株是否 小鼠对SV去除的反应不同， 去除SV后不同品系小鼠的生育力降低 与精子活力的改变有关 如果一组 具有不同程度的生育能力损害的菌株可以是 可以确定精子的哪些方面 运动能力与生育能力关系最为密切。 也可能是 可以使用这样一组动物来测试参数， 预测或治疗不孕症。 第三个目标是确定 是否SV的去除也会改变子宫精子凝集素的模式， 结合，并决定精子凝集素结合研究是否可以 可以用来预测生育率。 学生参与者在此 这个项目将学习视频显微分析技术 精子活力和制作染色切片的标准技术 组织和细胞。 他们也将接触到当前的想法 在生育研究中。 研究结果应能 我们对影响生育力的自然过程有了深入的了解 in vivo.