MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM

这项提案是对生物医学研究的拨款申请 研究部资助(BRS)赠款计划 资源(DRR)建立多用户设施以实现自动化 香豆素的合成、提纯及低温储藏 多肽。华盛顿大学生物医学研究生院 德克萨斯大学加尔维斯顿分校(UTMB)收到 来自BRS的支持，赠款#DHHS 2S07 RR 07205-05，因此 有资格申请BRS-DRR补助金。 要求为多肽合成器提供资金，这是一种高 高效液相色谱(HPLC)系统和低成本 提供多肽合成能力的温度冷冻器 14个有记录的正在进行的生物医学研究项目，其中大多数 目前由美国国立卫生研究院资助 (美国国立卫生研究院)。这些项目包括六个与分子有关的项目 配体-受体相互作用的基础，三个项目涉及 蛋白质结构和功能的免疫学解剖，三 涉及神经化学和特定性质的项目 多肽作为神经递质分子，一个项目涉及 膜保护免受自由基的伤害，以及一个项目 在人工合成中使用多肽作为离子通道修饰剂 膜。建议的多肽合成器的获得是 需要更有效地实现这些目标 NIH批准的项目并允许这些调查人员扩大 他们的研究努力跟上了取得的快速进展 通过使用合成多肽是可能的。 多肽合成器将被安置在旁边，并将 与集中式蛋白质化学联合运行 实验室。蛋白质化学实验室已经建立了 正在进行的共享检测使用的记录，并具有 建立了处理分摊费用的行政机制。 向院长负责的研究咨询委员会 研究生院将协助项目主任 多肽合成器的管理和科学评价 设施，以确保适当的共享和最大限度地利用 仪器仪表。UTMB政府已同意提供 空间，研究助理工资，并继续支持 运营此设施的服务，允许用户只收取费用 用来购买消耗品。

This proposal is a grant request to the Biomedical Research Support (BRS) grant program of the Division of Research Resources (DRR) to establish a multi-user facility for automated synthesis, purification and low temperature storage of polypeptides. The Graduate School of Biomedical Sciences of the University of Texas Medical Branch (UTMB) at Galveston receives support from BRS, grant # DHHS 2S07 RR 07205-05 and is thus eligible to apply for a BRS-DRR grant. Funding is requested for a peptide synthesizer, a high performance liquid chromatography (HPLC) system and a low temperature freezer to provide a peptide synthesis capability for 14 documented ongoing biomedical research projects, most of which are currently funded by the National Institutes of Health (NIH). These programs include six projects dealing with molecular basis of ligand-receptor interactions, three projects dealing with immunological dissection of protein structure and function, three projects involving neurochemistry and the nature of specific peptides as neurotransmitter molecules, one project involving membrane protection from free radicals, and one project involving the use of peptides as ion channel modifiers in artificial membranes. Acquisition of the proposed peptide synthesizer is required to more effectively meet the stated objectives of these NIH approved projects and allow these investigators to expand their research efforts to keep pace with the rapid progress made possible by the use of synthetic peptides. The peptide synthesizer will be housed adjacent to, and will be operated in conjunction with, a centralized Protein Chemistry Laboratory. The Protein Chemistry Laboratory has an established record of ongoing shared instrumentation usage and has an administrative mechanism in place for dealing with shared costs. A Research Advisory Committee responsible to the Dean of the Graduate School will assist the Program Director in administrative and scientific review of the peptide synthesizer facility to insure proper sharing and maximizing the use of this instrumentation. The UTMB administration has agreed to provide space, a Research Associate salary, and continued support for service to operate this facility allowing users to be charged only for consumable supplies.