Live cell super-resolution microscopy assessment of targeted unimolecular nanomachines

靶向单分子纳米机器的活细胞超分辨率显微镜评估

• 批准号: EP/P025684/1
• 负责人: Robert Pal
• 金额: $ 0.21万
• 依托单位: Durham University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FP025684%2F1
• 关键词: Live; cell; super; resolution; microscopy

This proposal seeks to cement the scientific collaboration further between Prof. Jim Tour and Dr. Robert Pal. Our combined research aim is to develop and test small molecular agents that once activated by low dosage of Ultra-violet light can selectively destroy cancerous cells and tissues.This collaboration has been established, when Prof. Tour visited Durham as the 2015 Durham Lecturer at our department. Prof. Tour proposed a, yet unanswered, scientific challenge as part of one of his lectures, that due to my research expertise and available instrumentation I was able to propose a series of experiments to answer with. Exactly one year and lots of hard work later now we are in the fortunate position that our collective findings have been submitted to Nature and is currently under review of publication. As a result of this well-oiled collaboration not only this mutually beneficial milestone have been met, but a further more extensive NIH research grant have been submitted 12/2016 to facilitate the synthesis of the next generation of complexes to be studied at Durham (decision pending). However, the above mentioned proposal would only facilitate research staff and consumables cost to be met at Rice University. Up to this date Dr. Pal as a Royal Society URF, who as a URF is severely limited to apply for funding to undertake such activity, had no associated funding to visit Prof. Tour's lab, a visit that would facilitate mutual knowledge exchange and the establishment of a suitable optical microscopy facility to conduct any required preliminary screening of active compounds before the subsequent Durham live cell evaluation. There is no better way to showcase the scientific timeliness and magnitude of our combined research area than the 2016 Nobel Prize in chemistry, that has been awarded for 'Molecular Machines', and the 2014 Nobel Chemistry Prize what has been awarded for 'Super-resolution microscopy'. This small travel grant could facilitate this and accelerate the future success of the project greatly.

该提案旨在进一步巩固Jim Tour教授和Robert Tour博士之间的科学合作。我们的联合研究目标是开发和测试小分子药物，一旦被低剂量的紫外线激活，可以选择性地破坏癌细胞和组织。这种合作已经建立，当教授旅游访问达勒姆作为2015年达勒姆讲师在我们部门。图尔教授在他的一次演讲中提出了一个尚未得到解答的科学挑战，由于我的研究专业知识和可用的仪器，我能够提出一系列实验来回答。整整一年，经过大量的艰苦工作，我们现在处于幸运的位置，我们的集体发现已经提交给自然，目前正在审查出版。由于这种良好的合作，不仅实现了这一互利的里程碑，而且还于2016年12月提交了进一步更广泛的NIH研究资助，以促进在达勒姆研究的下一代复合物的合成（决定待定）。然而，上述建议只会促进研究人员和消耗品的费用，以满足在莱斯大学。迄今为止，Dr. Wendy作为皇家学会URF（作为URF，他申请开展此类活动的资金受到严格限制）没有相关资金访问Tour教授的实验室，该访问将促进相互知识交流并建立合适的光学显微镜设施，以便在随后的达勒姆活细胞评价之前对活性化合物进行任何必要的初步筛选。有没有更好的方式来展示我们的综合研究领域的科学及时性和规模比2016年诺贝尔化学奖，已被授予“分子机器”，2014年诺贝尔化学奖已被授予“超分辨率显微镜”。这笔小额旅费赠款可以促进这一点，并大大加快该项目未来的成功。