Live cell super-resolution microscopy assessment of novel ratiometric luminescent transition metal complexes.

该提案旨在加强和提供申请人与泰国彭世洛那瑞宣大学Filip Kielar博士目前正在进行的合作中的下一个重要里程碑。此次合作是基于Filip Kielar博士在过渡金属络合物领域的专业知识和Robert Dallas博士在光学显微镜和相关活细胞成像和时间分辨光谱学方面的专业知识。由于过渡金属配合物的独特性质，例如与短寿命的组织自发荧光相反的较长荧光寿命以及它们作为光诱导单线态氧发生器的潜在应用，它们在细胞中的应用引起了极大的兴趣。我们建议研究这些新合成的化合物，这些化合物可以使用各种光学技术，如激光扫描共聚焦，时间分辨-这项合作最初是由于2014年皇家学会国际交流计划的资助而建立的，该计划授予了Dr. Dinner和Dr Kielar，使他们能够参与协作工作。该计划是一项旅行补助金，允许他们每人进行为期2周的访问，促进所有重要的面对面会议和讨论，以塑造和推动项目向前发展，最大限度地提高科学产出，并提供研究生和研究生讲座。整整一年，经过大量的艰苦工作，我们处于幸运的位置，我们的集体发现已发表在道尔顿交易（2016，45，17420）。由于这种良好的合作，2016年12月提交了一份更广泛的泰国研究基金赠款，以促进在达勒姆研究的下一代复合物的合成（授予Kielar博士，2017年2月开始，PI没有旅行津贴）。然而，上述补助金仅用于帮助Naresuan大学的研究人员（3年博士）和消耗品费用。Dr. Jesus已经将该项目的继续指定为2017/18年的专用硕士学位。学生。Narressuan大学的研究人员对我们在这个问题上初步富有成效的合作工作和交流感到非常高兴，并真正相信，由于达勒姆的专业知识和奉献精神，我们在这么短的时间内在这个问题上取得了这么多的成就，尽管物流源于我们非常不同的地质和财务状况。

This proposal is aimed at strengthening and providing the all-important next milestone in the current ongoing collaboration between the applicant and Dr. Filip Kielar, Naresuan University, Phitsanulok, Thailand. The collaboration is based on the Dr. Filip Kielar's expertise in the field of transition metal complexes and the expertise of Dr. Robert Pal in optical microscopy and associated live cell imaging and time-resolved spectroscopy. Due to the unique nature of transition metal complexes, such as longer fluorescent lifetime as opposed to short lived tissue auto-fluorescence and their potential application as photoinduced singlet oxygen generators, their in cellulo application is of great interest. We propose to study these newly synthesized compounds that can report on key biologically important analytes in live-cell studies using a variety of optical techniques, such as Laser Scanning Confocal-, Time-resolved- and Fluorescent Life Time imaging Microscopy.This collaboration was initially established as a result of a Royal Society International Exchange Scheme grant in 2014 that was awarded to Dr. Pal and Dr Kielar allowing them to engage in collaborative work. The scheme, being a travel only grant, allowed them to conduct a 2 week long visit each, facilitating all-important face to face meetings and discussions to shape and drive the project forward maximising scientific output and delivering under and post graduate lectures. Exactly one year and lots of hard work later we were in the fortunate positon, that our collective findings have been published in Dalton Transactions (2016, 45, 17420). As a result of this well-oiled collaboration a further more extensive Thai Research Fund grant was submitted in 12/2016 to facilitate the synthesis of the next generation of complexes to be studied at Durham (awarded to Dr. Kielar, commenced 02/2017 with no travel allowance for the PI). However, the above mentioned grant only facilitates research staff (3 year PhD) and consumables cost to be met at Naresuan University. Dr. Pal has already earmarked the continuation of this project as a dedicated 2017/18 MSc. student.Researchers at Narressuan University are extremely happy with our preliminary fruitful collaborative work and communications on this subject, and truly believe that due to the expertise and dedication at Durham we have achieved so much in so little time on this subject despite the logistics originating from our very different geological and financial position.