PH AND THE HEAVY METALS ALUMINUM, COPPER, LEAD, AND ZINC

pH 值和重金属铝、铜、铅和锌

• 批准号: 3778100
• 负责人: JOSEPH RACHLIN
• 金额: --
• 依托单位: HERBERT H. LEHMAN COLLEGE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3778100
• 关键词: acidity /alkalinity; algae; aluminum; chlorophyll; copper; fresh water environment; intracellular; lead; metal poisoning; minority institution research support; plant growth /development; plant morphology; scanning electron microscopy; tissue /cell culture; zinc

It is the purpose of this investigation to document the effects of changing pH and Al, Cu, Pb and Zn toxicity on freshwater lake algae. We will focus the study on cellular changes and the growth rates of the algae at different pH in the presence and absence of Al, Cu, Pb and Zn. Specifically, the objectives of this study are: 1. Determine the effect of changing pH from 9.0 to 3.0 on the growth of selected representative freshwater lake algae. 2. Determine the EC50 of Al, cu, Pb and Zn at the different pHs used in this study. 3. Determine cellular effects using morphometry at the different pHs tested and the effect of Al, Cu, Pb and Zn at the EC50 and one log concentration above and below this point. 4. Determine the effect of each treatment on the chlorophyll content of the cells. This is a good estimate of productivity. 5. Determine using X-ray energy dispersive analysis with a scanning transmission electron microscope if compartmentalization of the Al, Cu, Pb, and Zn has occurred. 6. To establish the toxicity hierarchy among these four cations at the varying pH levels in order to determine the importance of Al as a key toxicant in acidogenesis. It is felt, in order to understand the toxic effects of heavy metals to cell systems, that it is necessary to document how pH alterations effect the mobilization of these metals at the cellular and intracellular level. This project is designed to address this question.

这项调查的目的是记录改变的影响 pH和Al，Cu，Pb和Zn毒性在淡水湖藻类上的毒性。 我们将集中精力 细胞变化和藻类生长速率的研究 在存在和不存在Al，Cu，Pb和Zn的情况下，pH值不同。 具体而言，这项研究的目标是： 1。确定将pH从9.0更改为3.0对生长的影响 选定的代表淡水湖藻类。 2。确定Al，Cu，Pb和Zn的EC50在使用的不同pHS上 这项研究。 3。在测试的不同pHS上使用形态计算确定细胞效应 以及Al，Cu，Pb和Zn在EC50和一个对数集中的效果 在这一点上方和下方。 4。确定每种治疗对叶绿素含量的影响 细胞。 这是对生产率的良好估计。 5。通过扫描确定使用X射线能量色散分析 透射电子显微镜如果Al，Cu，Pb， Zn发生了。 6。在这四个阳离子之间建立毒性层次结构 为了确定AL作为关键的重要性而变化的pH值 在酸中有毒。 人们认为，为了了解重金属的毒性作用 细胞系统，有必要记录pH变化的效果 这些金属在细胞和细胞内水平的动员。 该项目旨在解决这个问题。