PH AND THE HEAVY MNETALS AL, CU, PB AND ZN

这项调查的目的是记录 淡水湖pH变化与铝、铜、铅、锌毒性 藻类。我们将重点研究细胞的变化和生长 有无藻类在不同pH值下的藻类速率 铝、铜、铅和锌。具体来说，这项研究的目标是： 1.确定pH值从11.0改变到4.0对 选定具有代表性的淡水类藻类的生长。 2.测定铝、铜、铅、锌在不同pH值下的EC50 在这项研究中使用。 3.使用形态计量学方法确定不同区域的细胞效应 PHS测试及铝、铜、铅、锌在EC50和1的影响 在这一点上方和下方记录浓度。 4.测定各处理对叶绿素的影响 单元格的内容。这是对生产率的一个很好的估计。 5.用X射线能量色散分析测定 扫描电子显微镜如果分隔化 出现了铝、铜、铅、锌元素的共生。 6.建立这四种阳离子的毒性等级 在不同的pH值水平下，以确定 铝是酸化过程中的关键毒物。 我们觉得迫切需要阐明酸的影响。 雨水落在我们的淡水湖上，正在慢慢地把它们变成酸 湖泊。一组看似缺乏但却至关重要的数据 我们对这个问题的评估是可靠的科学数据， 明确地展示了酸雨的建议影响，通过 固定化金属中pH的变化对藻类系统的影响。

It is the purpose of this investigation to document the effects of changing pH and Al, Cu, Pb and Zn toxicity on freshwater lake algae. We will focus the study on cellular changes and the growth rates of the algae at different pH in the presence and absence of Al, Cu, Pb and Zn. Specifically, the objectives of this study are: 1. Determine the effect of changing pH from 11.0 to 4.0 on the growth of selected representative freshwater like algae. 2. Determine the EC50 of Al, Cu, Pb and Zn at the different pHs used in this study. 3. Determine cellular effects using morphometry at the different pHs tested and the effect of Al, Cu, Pb and Zn at the EC50 and one log concentration above and below this point. 4. Determine the effect of each treatment on the chlorophyll content of the cells. This is a good estimate of productivity. 5. Determine using X-ray energy dispersive analysis with a scanning transmission electron microscope if compartmentalization of the Al, Cu, Pb and Zn has occurred. 6. To establish the toxicity hierarchy among these four cations at the varying pH levels in order to determine the importance of Al as a key toxicant in acidogenesis. We feel there is an urgent need to elucidate the impact of acid rain on our freshwater lakes which is slowly turning them into acid lakes. One set of data which seems to be lacking but is critical to our assessment of this problem is solid scientific data which unequivocally demonstrates the suggested effect of acid rain, via pH alterations in mobilized metal effects on algal systems.