PH AND THE HEAVY METALS ALUMINUM, COPPER, LEAD, AND ZINC

pH 值和重金属铝、铜、铅和锌

• 批准号: 3856643
• 负责人: JOSEPH RACHLIN
• 金额: --
• 依托单位: HERBERT H. LEHMAN COLLEGE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3856643
• 关键词: acidity /alkalinity; algae; aluminum; chlorophyll; copper; fresh water environment; intracellular; lead; metal poisoning; minority institution research support; plant growth /development; plant morphology; scanning electron microscopy; tissue /cell culture; zinc

It is the purpose of this investigation to document the effects of changing pH and Al, Cu, Pb and Zn toxicity on freshwater lake algae. We will focus the study on cellular changes and the growth rates of the algae at different pH in the presence and absence of Al, Cu, Pb and Zn. Specifically, the objectives of this study are: 1. Determine the effect of changing pH from 9.0 to 3.0 on the growth of selected representative freshwater lake algae. 2. Determine the EC50 of Al, cu, Pb and Zn at the different pHs used in this study. 3. Determine cellular effects using morphometry at the different pHs tested and the effect of Al, Cu, Pb and Zn at the EC50 and one log concentration above and below this point. 4. Determine the effect of each treatment on the chlorophyll content of the cells. This is a good estimate of productivity. 5. Determine using X-ray energy dispersive analysis with a scanning transmission electron microscope if compartmentalization of the Al, Cu, Pb, and Zn has occurred. 6. To establish the toxicity hierarchy among these four cations at the varying pH levels in order to determine the importance of Al as a key toxicant in acidogenesis. It is felt, in order to understand the toxic effects of heavy metals to cell systems, that it is necessary to document how pH alterations effect the mobilization of these metals at the cellular and intracellular level. This project is designed to address this question.

本次调查的目的是记录改变的影响 pH 值和 Al、Cu、Pb 和 Zn 对淡水湖藻类的毒性。 我们将重点 对细胞变化和藻类生长速率的研究 存在和不存在 Al、Cu、Pb 和 Zn 时的不同 pH 值。 具体来说，本研究的目标是： 1. 确定 pH 从 9.0 变为 3.0 对生长的影响 精选具有代表性的淡水湖藻类。 2. 确定 Al、cu、Pb 和 Zn 在不同 pH 值下的 EC50 这项研究。 3. 在测试的不同 pH 值下使用形态测定法确定细胞效应 以及 Al、Cu、Pb 和 Zn 在 EC50 和 1 个对数浓度下的影响 高于和低于该点。 4.确定每种处理对叶绿素含量的影响 细胞。 这是对生产率的良好估计。 5. 使用 X 射线能量色散分析和扫描进行确定 透射电子显微镜如果划分 Al、Cu、Pb、 和Zn发生了。 6. 建立这四种阳离子的毒性等级 改变 pH 值以确定 Al 作为关键的重要性 产酸过程中的毒物。 为了了解重金属对人体的毒性作用 细胞系统，有必要记录 pH 值变化如何影响 这些金属在细胞和细胞内水平的动员。 这个项目就是为了解决这个问题而设计的。