PH AND THE HEAVY MNETALS AL, CU, PB AND ZN

PH 和重金属 AL、CU、PB 和 ZN

• 批准号: 3916070
• 负责人: JOSEPH RACHLIN
• 金额: --
• 依托单位: HERBERT H. LEHMAN COLLEGE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3916070
• 关键词: acidity /alkalinity; algae; aluminum; chlorophyll; copper; electron microscopy; fresh water environment; heavy metals; lead; lead poisoning; metal poisoning; zinc

It is the purpose of this investigation to document the effects of changing pH and Al, Cu, Pb and Zn toxicity on freshwater lake algae. We will focus the study on cellular changes and the growth rates of the algae at different pH in the presence and absence of Al, Cu, Pb and Zn. Specifically, the objectives of this study are: 1. Determine the effect of changing pH from 11.0 to 4.0 on the growth of selected representative freshwater like algae. 2. Determine the EC50 of Al, Cu, Pb and Zn at the different pHs used in this study. 3. Determine cellular effects using morphometry at the different pHs tested and the effect of Al, Cu, Pb and Zn at the EC50 and one log concentration above and below this point. 4. Determine the effect of each treatment on the chlorophyll content of the cells. This is a good estimate of productivity. 5. Determine using X-ray energy dispersive analysis with a scanning transmission electron microscope if compartmentalization of the Al, Cu, Pb and Zn has occurred. 6. To establish the toxicity hierarchy among these four cations at the varying pH levels in order to determine the importance of Al as a key toxicant in acidogenesis. We feel there is an urgent need to elucidate the impact of acid rain on our freshwater lakes which is slowly turning them into acid lakes. One set of data which seems to be lacking but is critical to our assessment of this problem is solid scientific data which unequivocally demonstrates the suggested effect of acid rain, via pH alterations in mobilized metal effects on algal systems.

这项调查的目的是记录 更改pH和Al，Cu，Pb和Zn毒性在淡水湖上的毒性 藻类。 我们将把研究重点放在细胞变化和生长上 在存在和不存在的情况下，藻类在不同的pH下的速率 Al，Cu，Pb和Zn。 具体而言，这项研究的目标是： 1。确定将pH从11.0更改为4.0对 藻类等选定代表性淡水的生长。 2。在不同的pHS上确定Al，Cu，Pb和Zn的EC50 在这项研究中使用。 3。使用形态测定法确定细胞效应 PHS测试以及Al，Cu，Pb和Zn在EC50和1的效果 该点以上和下方的对数集中。 4。确定每种治疗对叶绿素的影响 细胞的含量。 这是对生产率的良好估计。 5。确定使用X射线能量色散分析 扫描透射电子显微镜如果分隔 发生了Al，Cu，Pb和Zn的发生。 6。在这四个阳离子中建立毒性层次结构 在不同的pH值以确定的重要性 Al作为酸生成中的关键毒物。 我们认为迫切需要阐明酸的影响 我们的淡水湖上的雨水正在慢慢变成酸 湖泊。 一组似乎缺少但至关重要的数据 对于我们对这个问题的评估是可靠的科学数据 明确地证明了酸雨的建议作用 动员金属对藻类系统的影响改变。