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BIOMEDICAL RESEARCH SUPPORT

生物医学研究支持

基本信息

批准号：
3518536
负责人：
MICHAEL V RILEY
金额：
$ 5万
依托单位：
OAKLAND UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1979
资助国家：
美国
起止时间：
1979-04-01 至 1993-09-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3518536
关键词：
health science research support university

项目摘要

This revised proposal requests funding for a laser scanning confocal microscope (LSCM) and appropriate accessories. The applicants are nine faculty members from the Departments of Anatomy, Growth and Development, Pharmaceutical Chemistry, Pharmacy, Physiology, and Stomatology (spanning the Schools of Dentistry, Medicine and Pharmacy). The research programs of the investigators require detailed information on the three-dimensional localization of molecules in extracellular and intracellular space. Projects are described which involve applications of LSCM both to whole tissue samples and to cellular and subcellular events. Specifically, the following areas of significance will be explored: epidermal barrier development and enhancement of percutaneous transport, migratory and invasive activity of early embryonic cells, tumor cell invasion of basement membrane and interstitial matrices; receptor-mediated endocytosis and the uptake of antigenic peptides, biochemical and biological characterization of transforming growth factors (TGF-alpha and TGF-beta), protein traffic in polarized epithelial cells, the regulatory mechanism(s) of neuronal nicotinic acetylcholine receptors, and the uptake and processing of oligonucleotides by cells in culture. For each research program outlined, the ability of the LSCM to analyze and quantify the spatial distribution of molecules through membranes, and within and around cells, is crucial. This quality of sophisticated information cannot be achieved with conventional immunofluorescence microscopy, nor can it be accurately assessed using electron microscopy. Considerable augmentation of multiple research directions will result, therefore, from the availability of a confocal microscope at this time. Although there are such instruments at UCSF, access for the present investigative team is very restricted and is becoming progressively more difficult. The School of Pharmacy administration has committed space for the location of a new apparatus in the event that this application is successful. We believe, therefore, that we have an important need for this shared equipment, that feasible alternatives are not available at our institution, and that a strong Departmental and School commitment to the long-term operation and maintenance of the LSCM is in place. The instrument requested is a BioRad- 600 laser-scanning confocal microscope. The overall system will consist of several interconnected components: confocal imaging system, standard epifluorescence microscope, video printer, and optical disk for mass storage.

这份修订后的提案要求为激光扫描共聚焦提供资金显微镜(LSCM)和适当的附件。申请者是9岁来自解剖、生长和发育系的教职员工，药物化学、药学、生理学和口腔医学(跨学科牙科、医学和药学学院)。的研究计划调查人员要求提供有关三维空间的详细信息分子在胞外和胞内空间的定位。描述了涉及LSCM在整体上的应用的项目组织样本以及细胞和亚细胞事件。具体地说，将探索以下重要领域：表皮屏障发展和加强经皮转运、迁移和早期胚胎细胞侵袭活性、肿瘤细胞侵袭基底膜和间质基质；受体介导的内吞作用抗原肽的摄取、生化和生物学特性转化生长因子(转化生长因子-α和转化生长因子-β)，蛋白流量在极化上皮细胞，神经元的调节机制(S) 烟碱型乙酰胆碱受体及其摄取和加工培养细胞中的寡核苷酸。对于每个概述的研究计划，土地供应链管理分析和量化空间分布的能力通过细胞膜以及细胞内和细胞周围的分子是至关重要的。这复杂信息的质量是传统技术无法实现的免疫荧光显微镜检查，也不能用电子显微镜。多项研究的显著增强因此，方向将根据共焦的可用性而产生显微镜在这个时候。尽管加州大学旧金山分校有这样的仪器，目前的调查小组的准入受到很大限制，变得越来越难。药学院政府已预留空间用于在中国放置新仪器此应用程序成功的事件。因此，我们认为，我们对这种共享设备有重要的需求，这是可行的我们的机构没有其他选择，而且有很强的部门和学校对长期运作的承诺和 LSCM的维护工作已经到位。所要求的仪器是BioRad- 600激光扫描共聚焦显微镜。整个系统将包括几个相互连接的组件：共焦成像系统，标准荧光显微镜、视频打印机和用于质量的光盘储藏室。