FERMENTATION EQUIPMENT

发酵设备

• 批准号: 3519212
• 负责人: ROY CURTISS III
• 金额: $ 8.71万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-12-01 至 1986-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3519212
• 关键词: antibacterial agents; bacterial genetics; bacterial vaccines; biotechnology; centrifugation; fermentation; immunological substance; microorganism culture; microorganism metabolism

Funds are requested to purchase a Queue LH Fermentor that permits controlled growth of microorganisms and a New Brunswick CEPA Z41 centrifuge for harvesting cells. The fermentor and centrifuge will be placed in a newly renovated fermentation facility. Other equipment to be in this facility and the renovation will be paid for by Washington University. These equipment items will facilitate research in the Curtiss lab to establish the genetic and biochemical bases for pathogenicity of various bacterial infectious disease agents in the genera Salmonella, Shigella, Streptococcus and Mycobacterium using a diversity of genetic (cloning, gene fusions, transposon mutagenesis, gene transfer, etc.), biochemical, immunological and microscopic techniques. Information learned from these basic studies will be used to develop novel means for vaccination, improved methods for early diagnosis and novel methods to screen for new drugs that might be effective in treating infections caused by bacterial agents. A specific goal of the vaccine development work is to enhance the secretory immune system so as to protect against infectious disease agents which colonize on or invade through a mucosal surface. Dr. Beachy's group will use the facility to recover Beta-galactosidase fusion proteins to serve as antigens specified by cloned cDNA whose mRNAs are regulated during the development of soybean embryos. Dr. Elgin's group will produce by recombinant techniques large quantities of non-histone chromosomal binding proteins that will be evaluated for ability to regulate developmental genes in Drosophila. Dr. Goodenough will grow Chlamydomonas to isolate the agglutinin responsible for sexual recognition and the ciliary protein dynein in order to analyze mating at the molecular level and the mechanism of ciliary motility, respectively. Dr. Silver's lab will use the fermentor to recover large quantities of proteins specified by the genes of the mercury-resistance operon to permit detailed structure-function studies including crystallographic analysis. Dr. Varner will use recombinant DNA techniques to permit production and recovery of large amounts of the proline-containing precursor of the hydroxyproline-rich glyco-protein of plant cell walls in order to study synthesis of the prolyl hydroxylase as a stress response in plants.

需要资金购买 Queue LH 发酵罐，该发酵罐允许 微生物的受控生长和新不伦瑞克 CEPA Z41 离心机 用于收获细胞。 将发酵罐和离心机放置在 新装修的发酵设施。 其他设备在此 设施和装修费用将由华盛顿大学支付。 这些设备项目将促进柯蒂斯实验室的研究 建立各种致病性的遗传和生化基础 沙门氏菌属、志贺氏菌属、 链球菌和分枝杆菌利用多种遗传（克隆、基因 融合、转座子诱变、基因转移等）、生物化学、 免疫学和显微镜技术。 从这些资料中了解到的信息 基础研究将用于开发新的疫苗接种方法，改进 早期诊断方法和筛选新药的新方法 可能有效治疗细菌引起的感染。 一个 疫苗研制工作的具体目标是增强分泌 免疫系统，以防止传染病病原体 定植于粘膜表面或通过粘膜表面侵入。 比奇博士的小组将 使用该设施回收 β-半乳糖苷酶融合蛋白作为 由克隆的 cDNA 指定的抗原，其 mRNA 在 大豆胚的发育。 埃尔金博士的小组将生产 重组技术大量非组蛋白染色体结合 将评估调节发育基因的能力的蛋白质 在果蝇中。 古迪纳夫博士将种植衣藻来分离 负责性别识别的凝集素和纤毛蛋白 动力蛋白以在分子水平上分析交配及其机制 分别是纤毛运动。 Silver 博士的实验室将使用发酵罐 回收大量由基因指定的蛋白质 耐汞操纵子可进行详细的结构功能研究 包括晶体学分析。 Varner 博士将使用重组 DNA 允许生产和回收大量的技术 富含羟脯氨酸的糖蛋白的含脯氨酸前体 植物细胞壁，以研究脯氨酰羟化酶的合成 植物的应激反应。