FLOW CYTOMETRY RESOURSE UPGRADE
流式细胞术资源升级
基本信息
- 批准号:3520022
- 负责人:
- 金额:$ 20.1万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1988
- 资助国家:美国
- 起止时间:1988-04-11 至 1989-04-10
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The FACS IV dual fluorescence/fifth parameter/computer upgrade
requested here will enable the Flow Cytometry Laboratory at The
University of South Alabama to perform the following functions
not now available with the present flow cytometry ste-up: 1)
simultaneous DNA and cell surface marker studies on viable cells,
2) two-color analyses using Texas Red as an alternative to
phycoerythrin, 3) three-color analyses using Texas Red or
allophycocyanin as the third fluorochrome together with FITC and
phycoerythrin, 4) list mode data storage, making possible the re-
analysis of samples, at a future date, using different gating
parameters; also, making possible the simultaneous acquisition
and storage of data from five parameters, 5) bivariate
chromosome analysis using the Hoechst stains together with
chromomycin A3, 6) contour plot, 3-D isometric, and multiple
histogram data displays, 7) time of flight, and 8) single cell
deposition for cloning and hybridoma screening. This upgraded
FACS IV/computer resource will be instrumental in providing new
capabilities for a variety of analytical and preparative cell
techniques in the following NIH funded projects: 1. The
characterization of oncofetal antigens, and monoclonal antibodies
to these antigens, expressed on human and rodent tumors. 2.
Analysis of a 44 kD oncofetal antigen on radiation induced
leukemias and thymomas in mice. 3. Analysis and purification of
parietal cells from the rat stomach. 4. Cell surface marker
analysis of monoclonal populations of T helper cells, T suppressor
cells and T contrasuppressor cells. 5. Phenotypic characterization
and purification of T cell clones specifically reactive with Herpes
simplex induced antigens. 6. Establishment of interferon-
resistant cell lines and analysis of cellular receptors for
interferon in the study of host defense against Rickettsiae. 7.
Effect of alloantigenic challenge on lymphocyte sub-set
composition in pregnancy and AIDS. 8. Isolation of insulin
secreting beta cells from the pancreas. 9. Investigation of
interferon-rickettsiae interactions using Rhodamine 123.
FACS IV双荧光/第五参数/计算机升级
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
RAYMOND B HESTER其他文献
RAYMOND B HESTER的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('RAYMOND B HESTER', 18)}}的其他基金
FACSVANTAGE SE UPGRADE: ENVIRONMENTAL HEALTH
FACSVANTAGE SE 升级:环境健康
- 批准号:
6973361 - 财政年份:2004
- 资助金额:
$ 20.1万 - 项目类别:
FACS 440 REPLACEMENT--INSURING CONTINUED FLOW CAPABILITY
FACS 440 更换——确保持续流动能力
- 批准号:
2503102 - 财政年份:1998
- 资助金额:
$ 20.1万 - 项目类别:
ACAS 570 ANCHORED CELL ANALYZER AND SORTER
ACAS 570 锚定细胞分析仪和分选仪
- 批准号:
3521216 - 财政年份:1991
- 资助金额:
$ 20.1万 - 项目类别: