FLOW CYTOMETRY RESOURSE UPGRADE

流式细胞术资源升级

基本信息

  • 批准号:
    3520022
  • 负责人:
  • 金额:
    $ 20.1万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1988
  • 资助国家:
    美国
  • 起止时间:
    1988-04-11 至 1989-04-10
  • 项目状态:
    已结题

项目摘要

The FACS IV dual fluorescence/fifth parameter/computer upgrade requested here will enable the Flow Cytometry Laboratory at The University of South Alabama to perform the following functions not now available with the present flow cytometry ste-up: 1) simultaneous DNA and cell surface marker studies on viable cells, 2) two-color analyses using Texas Red as an alternative to phycoerythrin, 3) three-color analyses using Texas Red or allophycocyanin as the third fluorochrome together with FITC and phycoerythrin, 4) list mode data storage, making possible the re- analysis of samples, at a future date, using different gating parameters; also, making possible the simultaneous acquisition and storage of data from five parameters, 5) bivariate chromosome analysis using the Hoechst stains together with chromomycin A3, 6) contour plot, 3-D isometric, and multiple histogram data displays, 7) time of flight, and 8) single cell deposition for cloning and hybridoma screening. This upgraded FACS IV/computer resource will be instrumental in providing new capabilities for a variety of analytical and preparative cell techniques in the following NIH funded projects: 1. The characterization of oncofetal antigens, and monoclonal antibodies to these antigens, expressed on human and rodent tumors. 2. Analysis of a 44 kD oncofetal antigen on radiation induced leukemias and thymomas in mice. 3. Analysis and purification of parietal cells from the rat stomach. 4. Cell surface marker analysis of monoclonal populations of T helper cells, T suppressor cells and T contrasuppressor cells. 5. Phenotypic characterization and purification of T cell clones specifically reactive with Herpes simplex induced antigens. 6. Establishment of interferon- resistant cell lines and analysis of cellular receptors for interferon in the study of host defense against Rickettsiae. 7. Effect of alloantigenic challenge on lymphocyte sub-set composition in pregnancy and AIDS. 8. Isolation of insulin secreting beta cells from the pancreas. 9. Investigation of interferon-rickettsiae interactions using Rhodamine 123.
FACS IV双荧光/第五参数/计算机升级

项目成果

期刊论文数量(0)
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会议论文数量(0)
专利数量(0)

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RAYMOND B HESTER其他文献

RAYMOND B HESTER的其他文献

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{{ truncateString('RAYMOND B HESTER', 18)}}的其他基金

FACSVANTAGE SE UPGRADE: LUNG
FACSVANTAGE SE 升级:肺
  • 批准号:
    6973359
  • 财政年份:
    2004
  • 资助金额:
    $ 20.1万
  • 项目类别:
FACSVANTAGE SE UPGRADE: ENVIRONMENTAL HEALTH
FACSVANTAGE SE 升级:环境健康
  • 批准号:
    6973361
  • 财政年份:
    2004
  • 资助金额:
    $ 20.1万
  • 项目类别:
FACSVANTAGE SE UPGRADE: CANCER
FACSVANTAGE SE 升级:癌症
  • 批准号:
    6973358
  • 财政年份:
    2004
  • 资助金额:
    $ 20.1万
  • 项目类别:
FACSVANTAGE SE UPGRADE: EYE
FACSVANTAGE SE 升级:眼睛
  • 批准号:
    6973360
  • 财政年份:
    2004
  • 资助金额:
    $ 20.1万
  • 项目类别:
FACSVANTAGE SE UPGRADE: CELL BIOLOGY
FACSVANTAGE SE 升级:细胞生物学
  • 批准号:
    6973362
  • 财政年份:
    2004
  • 资助金额:
    $ 20.1万
  • 项目类别:
FACSVantage SE Upgrade
FACSVantage SE 升级
  • 批准号:
    6731842
  • 财政年份:
    2004
  • 资助金额:
    $ 20.1万
  • 项目类别:
LEICA TCS SP SPECTRAL CONFOCAL MICROSCOPE
徕卡 TCS SP 光谱共焦显微镜
  • 批准号:
    6051652
  • 财政年份:
    2000
  • 资助金额:
    $ 20.1万
  • 项目类别:
FACS 440 REPLACEMENT--INSURING CONTINUED FLOW CAPABILITY
FACS 440 更换——确保持续流动能力
  • 批准号:
    2503102
  • 财政年份:
    1998
  • 资助金额:
    $ 20.1万
  • 项目类别:
ACAS 570 ANCHORED CELL ANALYZER AND SORTER
ACAS 570 锚定细胞分析仪和分选仪
  • 批准号:
    3521216
  • 财政年份:
    1991
  • 资助金额:
    $ 20.1万
  • 项目类别:
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