ACAS 570 ANCHORED CELL ANALYZER AND SORTER

ACAS 570 锚定细胞分析仪和分选仪

• 批准号: 3521216
• 负责人: RAYMOND B HESTER
• 金额: $ 29.48万
• 依托单位: UNIVERSITY OF SOUTH ALABAMA
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-02-05 至 1992-02-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521216
• 关键词: biomedical equipment purchase; confocal scanning microscopy; flow cytometry; image processing

The ACAS 570 interactive laser cytometer requested herein will provide the University of South Alabama College of Medicine with the capacity to perform the following functions not now available: (1) time- and spatially-resolved fluorescence analyses on individual cells, (2) the potential for studying cell-cell interactions at the level of the individual cells and to directly visualize the effects of such interactions using fluorescence image analysis, (3) isolation of anchored cells for enrichment, outgrowth and additional study without subjecting the cells to detachment protocols, (4) fluorescence redistribution after photobleaching to analyze inter- and intracellular translocation of membrane components, cytoplasmic structures and biologically-relevant molecules, (5) confocal imaging of cells and intracellular structures to precisely locate antigen- or other probe-associated fluorescence. The ACAS 570 will be instrumental in providing new capabilities for a variety of analytical and preparative cell techniques in the following NIH-funded projects: (1) endothelial cell (EC) prostacylin synthesis and red cell-EC interactions, (2) lung fibroblast (LF) heterogeneity and type II alveolar cell-LF interaction, (3) pulmonary surfactant recycling by type II epithelial cells, (4) prolactin and secretogranin distribution and associated intracellular parameters in a pituitary tumor-cell model system, (5) regulation of iron absorption at the single cell level, (6) mitochondria translocation in neurites, (7) intracellular biochemical parameters associated with sickling of red blood cells, (8) oxygen radicals in neutrophils, (9) eosinophil peroxidase regulation, (10) the role of the poly(ADP-ribose) system in chemically-induced beta cell damage, (11) calcium transport in diabetic cardiomyopathies, (12) Na-Ca exchange in immature myocardium, (13) epidermal morphogenesis. Three additional projects, not NIH funded, deal with: (1) an analysis of calcium-modulated proteins, S100 and calmodulin in cultured glial cells, (2) the regulation of calcium transients in gastric parietal cells, and (3) the characterization of intracellular spiroplasma infection in a neuroblastoma cell line. Collectively, these projects will be conducted in eight different departments within the College of Medicine and will represent at least 16 different experimental approaches to important biological problems that cannot be pursued without the technological support of the ACAS 570. The instrumentation will enable these and other investigators at this institution to explore frontier arenas of science with the most sophisticated instrumentation available today.

这里要求的ACAS 570交互式激光细胞仪将提供 南阿拉巴马大学医学院有能力 执行以下目前不可用的功能：(1)时间和 单个细胞的空间分辨荧光分析，(2) 在细胞水平上研究细胞间相互作用的可能性 并直接可视化这种相互作用的影响 利用荧光图像分析，(3)锚定细胞的分离 培养，生长和额外的研究，而不使细胞 分离方案，(4)光漂白后的荧光重分布 为了分析膜成分在细胞间和细胞内的转移， 细胞质结构和生物相关分子，(5)共聚焦 对细胞和细胞内结构进行成像以精确定位抗原- 或其他与探针相关的荧光。ACAS 570将发挥重要作用 在为各种分析和准备工作提供新能力方面 NIH资助的下列项目中的细胞技术：(1)内皮细胞 (EC)前列环素合成和红细胞-EC相互作用，(2)肺 成纤维细胞的异质性和肺泡II型细胞与成纤维细胞的相互作用 肺表面活性物质在II型上皮细胞中的循环，(4)催乳素 和分泌颗粒素的分布及相关的细胞内参数 垂体瘤-细胞模型系统，(5)铁吸收的调节 单细胞水平，(6)神经突内线粒体易位，(7) 与红细胞病态有关的细胞内生化指标 细胞，(8)中性粒细胞中的氧自由基，(9)嗜酸性粒细胞过氧化物酶 调节，(10)聚(ADP-核糖)系统在 化学诱导的β细胞损伤，(11)糖尿病患者的钙转运 心肌病，(12)未成熟心肌的钠-钙交换，(13) 表皮形态发生。另外三个项目，不是NIH资助的，达成协议 与：(1)钙调节蛋白、S100和钙调蛋白的分析 在培养的神经胶质细胞中，(2)钙瞬变的调节 胃壁细胞；(3)细胞内特征 神经母细胞瘤细胞系中的螺旋体感染。总而言之，这些 项目将在八个不同的部门内进行 并将代表至少16个不同的实验 解决重要生物学问题的方法 ACAS 570的技术支持。该工具将启用 这些人和这个机构的其他调查人员一起探索前沿 拥有最先进仪器的科学竞技场 今天。