FACSVantage SE Upgrade

FACSVantage SE 升级

• 批准号: 6731842
• 负责人: RAYMOND B HESTER
• 金额: $ 22.64万
• 依托单位: UNIVERSITY OF SOUTH ALABAMA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6731842
• 关键词: biomedical equipment purchase; biomedical equipment resource; cell biology; flow cytometry; health science research; lasers

DESCRIPTION (provided by applicant): This proposal, if funded, will make possible an upgrade of our Becton Dickinson FACSVantage SE flow cytometer to include DiVa electronics, TurboSort, and the replacement of fifteen-year old lasers. The projects included in this application are being conducted with faculty in five departments within the University of South Alabama (USA) College of Medicine (COM) including the Departments of Microbiology/Immunology, Biochemistry and Molecular Biology, Cell Biology and Neuroscience, Pharmacology and Medicine, as well as the USA Cancer Research Institute, the Center for Lung Biology and the Sickle Cell Center. Nine of the projects involve NIH-funded investigators. The technology and reliability represented by this upgrade are required in the following areas of research in the USA COM: 1) repair of DNA induced by environmental agents, 2) the role of immature laminin receptor protein (iLRP) in tumor development and immune cell activation, 3) prostate epithelial cell-specific gene regulation, 4) regulation of cell cycle progression by ser/thr protein phosphatases, 5) antibody-mediated protection against herpes stromal keratitis and the role of chemokines in corneal inflammation, 6) cellular signaling in lymphocytes from New World primates, 7) metastasis-related antigen expression on human cancer cells and mechanisms of tumor cell sensitivity to therapy, 8) the reversal of inheritable mitochondrial disorders, NARP (neuropathy, ataxia, retinitis pigmentosa) and MILS (maternally inherited Leigh Syndrome), 9) role of ornithine decarboxylase in the endothelial cell toxin, monocrotaline, 10) role of activated leukocyte cell adhesion molecule and activated monocytes in the pathogenesis of acute chest pain, 11) role of erythrocyte-endothelial cell adhesion in acute lung injury in sickle cell disease, 12) chrondrocyte viability in osteochondrial tissue transfer, 13) acute lung injury in sickle cell disease and the role of erythrocyte-endothelial cell adhesion, 14) studies on metastasis-related antigen expression on human cancer cells, and 15) studies in immunotherapy in cancer patients. There has been an increasing awareness on the part of our NIH-funded investigators to require sort rates faster than those available with our Vantage SE as it is now configured. In addition, the lasers purchased with our FACS 440 in 1988, and transferred to the Vantage SE in 1998, are approaching the end of their useful lifespan as repairs becoming increasingly expensive and parts scarce. Four-way sorting (and increased) sort yields through the elimination of electronic dead-time), post acquisition compensation, greater flexibility with regard to signal triggering, and better data resolution, which are features of the DiVa option, will benefit all the members of our user group.

描述(申请人提供)：这项提案，如果获得资金，将使我们的Becton Dickinson FACSVantage SE流式细胞仪升级成为可能，包括DIVA电子设备、TurboSort和更换已有15年历史的激光。本申请中包含的项目是与南阿拉巴马大学医学院(COM)内五个系的教职员工共同实施的，包括微生物学/免疫学、生物化学和分子生物学、细胞生物学和神经科学、药理学和医学，以及美国癌症研究所、肺生物学中心和镰刀细胞中心。其中九个项目涉及美国国立卫生研究院资助的调查人员。这一升级所代表的技术和可靠性在美国COM的下列研究领域中是需要的：1)环境因素诱导的DNA修复，2)未成熟的层粘连蛋白受体蛋白(ILRP)在肿瘤发展和免疫细胞激活中的作用，3)前列腺上皮细胞特异性基因调控，4)Ser/Thr蛋白磷酸酶对细胞周期进程的调节，5)抗体介导的对疱疹间质角膜炎的保护和趋化因子在角膜炎症中的作用，6)新大陆灵长类动物淋巴细胞的细胞信号转导，7)人类癌细胞上转移相关抗原的表达和肿瘤细胞对治疗的敏感性机制，8)遗传性线粒体疾病，NARP(神经病变，共济失调，视网膜色素变性)和MILS(母系遗传性Leigh综合征)的逆转，9)鸟氨酸脱羧酶在内皮细胞毒素中的作用，野百合碱，10)激活的白细胞黏附分子和激活的单核细胞在急性胸痛的发病机制中的作用，11)红细胞与内皮细胞黏附在镰状细胞疾病的急性肺损伤中的作用，12)骨线粒体组织转移中的慢性细胞活性，13)镰状细胞疾病中的急性肺损伤以及红细胞与内皮细胞黏附的作用，14)关于人类癌细胞转移相关抗原表达的研究，以及15)在癌症患者的免疫治疗中的研究。我们NIH资助的研究人员越来越意识到，我们要求的分拣速度比我们的Vantage SE现在配置的分拣速度更快。此外，1988年随我们的FACS440购买的激光器，并于1998年转移到Vantage SE，由于维修费用越来越高，零部件越来越稀缺，其使用寿命即将结束。四向分拣(并通过消除电子死区而提高了分拣效率)、采集后补偿、在信号触发方面更大的灵活性以及更好的数据分辨率，这些都是DIVA选项的特点，将使我们用户组的所有成员受益。