FACS 440 REPLACEMENT--INSURING CONTINUED FLOW CAPABILITY

FACS 440 更换——确保持续流动能力

• 批准号: 2503102
• 负责人: RAYMOND B HESTER
• 金额: $ 21.16万
• 依托单位: UNIVERSITY OF SOUTH ALABAMA
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 1999-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2503102
• 关键词: biomedical equipment; biomedical equipment purchase; cell sorting; flow cytometry

This proposal, if funded, will make possible the purchase of a Becton Dickinson FACS Vantage cell sorter by the University of South Alabama (USA) College of Medicine (COM). The technology and reliability represented by this instrument are required in the following areas of research at USA COM: (1) mechanisms of tumor promotion and carcinogenesis, (2) the effects of aging on DNA repair, (3) DNA vaccines for Schistosomiasis, (4) oncofetal antigens as immunogenic markers of premalignancy or neoplasia in human and other mammalian species, (5) analysis of chemokines and cytokines in the inflamed cornea following Herpes simplex infection, (6) gene therapy for hemo-globinopathies and thalessemias, (7) cell adhesion molecules during vaso-occlusive episodes of sickle cell patients, (8) hypoxic pulmonary hypertension and polyamines, (9) cell cycle control of DNA nuclear import, (10) oxidative DNA modification and gene expression in hypoxia, (11) isolation of mutants of Salmonella typhimurium defective in acid tolerance, (12) isolation of Rickettsia prowazekii transformants in a continuing study to establish a genetic system in the rickettsiae, (13) analysis of mitochondrial membrane potential in glial cell populations, (14) purification of differentiated oligodendrocytes from postnatal murine brain for primary culture, (15) measurement of calcium transients in vascular smooth muscle cells, and (16) These projects are being conducted in seven different departments within the COM including Internal Medicine, Micro-biology and Immunology, Physiology, Pharmacology, Structural and Cellular Biology, Biochemistry and Molecular Biology, and Pediatrics. Eleven of the projects in this proposal are NIH-funded. The cell sorter now used by the USA COM, a FACS 440, has become increasingly difficult and expensive to repair. Spare parts are becoming scarce for the outdated technology. To insure continued availability of standard flow cytometric methodology, as well as acquisition of new and/or enhanced flow cytometry techniques, for the NIH-funded investigators at USA COM, will require the replacement of our FACS 440 with an updated version of this instrument.

这项建议，如果资金到位，将有可能购买一个Becton 南亚拉巴马大学的Dickinson FACS Vantage细胞分选仪 (USA)医学院（COM）。 技术和可靠性 在下列领域需要本文书所代表的 美国COM的研究：（1）肿瘤促进机制， 致癌作用，（2）衰老对DNA修复的影响，（3）DNA疫苗 对于血吸虫病，（4）癌胚抗原作为血吸虫病的免疫原性标志物， 人类和其他哺乳动物物种的癌前病变或瘤形成，（5） 炎症角膜中趋化因子和细胞因子的分析 单纯疱疹病毒感染，（6）血红蛋白病的基因治疗， 地中海贫血，（7）血管闭塞发作期间的细胞粘附分子 镰状细胞病患者，（8）缺氧性肺动脉高压， 多胺，（9）DNA核输入的细胞周期控制，（10）氧化 缺氧中的DNA修饰和基因表达，（11）分离 鼠伤寒沙门氏菌耐酸性缺陷突变体，（12） 普氏立克次体转化子分离 建立立克次体的遗传系统，（13）分析 神经胶质细胞群中的线粒体膜电位，（14） 从出生后小鼠中纯化分化的少突胶质细胞 用于原代培养的脑，（15）在脑中测量钙瞬变， 血管平滑肌细胞，和（16）这些项目正在进行 在COM内的七个不同部门进行，包括 内科学，微生物学和免疫学，生理学， 药理学、结构和细胞生物学、生物化学和 分子生物学和儿科。其中11个项目 该提案由NIH资助。 美国COM现在使用的细胞分类器， FACS 440的维修变得越来越困难和昂贵。 这种过时的技术的备件越来越少。 以确保 标准流式细胞术方法的持续可用性，以及 随着新的和/或增强的流式细胞术技术的获得， NIH资助的研究人员在美国COM，将需要更换我们的 FACS 440，该仪器的更新版本。