LEICA TCS SP SPECTRAL CONFOCAL MICROSCOPE

徕卡 TCS SP 光谱共焦显微镜

• 批准号: 6051652
• 负责人: RAYMOND B HESTER
• 金额: $ 33.68万
• 依托单位: UNIVERSITY OF SOUTH ALABAMA
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2002-05-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6051652
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; confocal scanning microscopy

Ten NIH-funded investigators at the University of South Alabama College of Medicine are seeking funds to obtain Leica microscope is justified on the basis of eminent need to replace an eight year old Meridian Instruments ACAS 570 that is becoming obsolete. Upgrading the ACAS is not a viable option since the ACAS is no longer manufactured and Meridian Instruments is out of business. The proposed confocal imaging system offers several needed imaging capabilities that our existing instrument lacks including: 1) faster image acquisition rates, 2) simultaneous use of 3 lasers, 3) increased spatial resolution, and 4) simultaneous monitoring of 3 wavelengths of emitted light via a spectrophotometric non-filter based detection system. The imaging system will be configured around a Leica DMR upright stand equipped for laser based differential interference contrast (DIG) microscopy. Illumination will be provided by 3 lasers: argon, argon/krypton and helium/neon. The Leica TCS 5P will support an initial user group of investigators in the areas of Lung Pathobiology, Cardiovascular Sciences, Neuroscience and Cancer/Oxidative Stress. It is anticipated that this group will use the instrument approximately 70% of the time. The confocal microscope will be housed in the College of Medicine Research Cytometry Core Laboratory which is under the direction of Dr. Raymond Hester. Administrative oversight and 1he operating budget for the Research Cytometry Core Laboratory are provided by the Senior Associate Dean of the College of Medicine. Investigators will pay a nominal fee for the use of the Leica TCS 5P. The addition of this new generation confocal microscope will provide NIH-funded investigators on our campus with state-of-the-art imaging capabilities to support their research.

南阿拉巴马大学医学院的10名NIH资助的研究人员正在寻求资金，以获得徕卡显微镜是合理的，因为迫切需要更换已有8年历史的子午线仪器ACAS 570，该仪器即将过时。升级ACAS不是一个可行的选择，因为ACAS不再生产，子午线仪器也停业了。拟议的共焦成像系统提供了我们现有仪器所缺乏的几种所需的成像功能，包括：1)更快的图像采集速度，2)同时使用3个激光，3)提高空间分辨率，以及4)通过基于分光光度的非滤光片检测系统同时监控3种波长的发射光。成像系统将配置在徕卡DMR直立支架周围，该支架配备了基于激光的差分干涉对比(DIG)显微镜。照明将由3个激光器提供：Ar、Ar/Krip和He/Ne。徕卡TCS 5P将支持肺部病理生物学、心血管科学、神经科学和癌症/氧化应激领域的研究人员的初始用户群。预计这一群体将在大约70%的时间内使用该仪器。共焦显微镜将被安置在医学研究学院细胞学核心实验室，该实验室由雷蒙德·海斯特博士领导。研究细胞学核心实验室的行政监督和业务预算由医学院高级副院长提供。调查人员将象征性地支付徕卡TCS 5P的使用费。新一代共聚焦显微镜的加入将为我们校园中由NIH资助的研究人员提供最先进的成像能力，以支持他们的研究。