LEICA TCS SP SPECTRAL CONFOCAL MICROSCOPE

南亚拉巴马大学医学院的10名NIH资助的研究人员正在寻求资金，以获得徕卡显微镜是合理的，因为他们迫切需要更换已经过时的8年前的子午线仪器ACAS 570。升级ACAS不是一个可行的选择，因为ACAS已不再生产，Meridian Instruments已停业。所提出的共焦成像系统提供了我们现有仪器所缺乏的几种所需的成像能力，包括：1）更快的图像采集速率，2）同时使用3个激光器，3）增加的空间分辨率，以及4）通过基于分光光度计的非滤光器检测系统同时监测3个波长的发射光。成像系统将围绕Leica DMR直立支架进行配置，该支架配备了基于激光的微分干涉对比（DIG）显微镜。照明将由3种激光器提供：氩、氩/氪和氦/氖。Leica TCS 5 P将为肺部病理生物学、心血管科学、神经科学和癌症/氧化应激领域的初始用户组提供支持。预计该组将在大约70%的时间内使用该仪器。共聚焦显微镜将被安置在医学研究细胞计数核心实验室的雷蒙德·赫斯特博士的指导下。细胞计数研究核心实验室的行政监督和运营预算由医学院高级副院长提供。研究者将为Leica TCS 5 P的使用支付象征性费用。新一代共聚焦显微镜的加入将为我们校园内NIH资助的研究人员提供最先进的成像能力，以支持他们的研究。

Ten NIH-funded investigators at the University of South Alabama College of Medicine are seeking funds to obtain Leica microscope is justified on the basis of eminent need to replace an eight year old Meridian Instruments ACAS 570 that is becoming obsolete. Upgrading the ACAS is not a viable option since the ACAS is no longer manufactured and Meridian Instruments is out of business. The proposed confocal imaging system offers several needed imaging capabilities that our existing instrument lacks including: 1) faster image acquisition rates, 2) simultaneous use of 3 lasers, 3) increased spatial resolution, and 4) simultaneous monitoring of 3 wavelengths of emitted light via a spectrophotometric non-filter based detection system. The imaging system will be configured around a Leica DMR upright stand equipped for laser based differential interference contrast (DIG) microscopy. Illumination will be provided by 3 lasers: argon, argon/krypton and helium/neon. The Leica TCS 5P will support an initial user group of investigators in the areas of Lung Pathobiology, Cardiovascular Sciences, Neuroscience and Cancer/Oxidative Stress. It is anticipated that this group will use the instrument approximately 70% of the time. The confocal microscope will be housed in the College of Medicine Research Cytometry Core Laboratory which is under the direction of Dr. Raymond Hester. Administrative oversight and 1he operating budget for the Research Cytometry Core Laboratory are provided by the Senior Associate Dean of the College of Medicine. Investigators will pay a nominal fee for the use of the Leica TCS 5P. The addition of this new generation confocal microscope will provide NIH-funded investigators on our campus with state-of-the-art imaging capabilities to support their research.