Multiscale characterization of complex materials using a combination of atomic force microscopy and optical coherence tomography

结合原子力显微镜和光学相干断层扫描对复杂材料进行多尺度表征

• 批准号: EP/R025606/1
• 负责人: Jinju Chen
• 金额: $ 62.06万
• 依托单位: Newcastle University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FR025606%2F1
• 关键词: Multiscale; characterization; complex; materials; using

Newcastle University (NCL) has a history of multidisciplinary collaborations in investigator-led research projects and in largescale, strategic initiatives such as the EPSRC Frontier Award (Oct 2013-June 2019, which involves researchers from four different schools within Newcastle University).The NCL capital equipment strategies are designed to ensure long-term access to equipment and computing facilities that support our diverse research needs from routine characterisation to state-of-the-art technique development. We recognise that sharing of scientific equipment between Newcastle and widely across the N8 partner universities (eight most research intensive Universities in the North of England) brings multiple benefits, including increased utilisation, greater collaboration, enhanced sustainability and a wider range of facilities available to our researchers.We have identified the proposed equipment that are required to underpin research in ESPRC priority areas in Materials Characterisation, Functional Materials, Engineering for Life and Health, Engineering Science, Water, Synthetic Biology, Regenerative Medicine, and Nanomedicine. This will benefit multiple research groups across NCL as well as external users both academic and industrial. It supports a portfolio of existing EPSRC grants and brings new capabilities to enable new scientific discoveries. A brief summary of the equipment requested and their functions are as follows:1). A cutting-edge AFM integrated with microfluidics, allows unprecedented possibilities for exciting applications in single-cell biology and nanoscience, thanks to the precise force control provided by the AFM and the microfluidics capabilities. The capability of high resolution and high accuracy imaging and mechanical measurements supports a range of projects in chemistry and at its interfaces with biology, physics and chemical engineering. It is complemented by the existing state-of-art high resolution surface chemistry analysis techniques available at Newcastle.2). An optical coherence tomography (OCT) uses infrared light to provide surface profiles and subsurface structures, delivering higher resolution and faster images than ultrasound inspection. When used together with the custom built air-pulse system, OCT also enables characterisation of elastic properties of materials such as biofilms and soft tissues. Items AFM and OCT together with the existing facilities at Newcastle permit mapping of 3D geometry and mechanical characterisation from sub-nanometer to millimetre scale. This asset enables the study of physical and dynamical properties of soft matter, facilitating investigations of disease progression at subcellular, cellular and tissue levels which will allow formulation of effective diseases diagnosis and treatment strategies. The multiscale quantitative measurement obtained by this asset can also provide datasets needed for constructing predictive models in healthcare and water engineering. As a consequence, it can underpin a range of academic and industry-focussed projects in marine biofilms, biological wastewater treatment, biofilm infections, tissue engineering, cancer research and many other healthcare related applications. Such multiscale physical and mechanical characterisation capability can be further reinforced by the advanced surface chemistry analysis facilities hosted at Newcastle, which will give rise to world-leading research in materials characterisation. It will enhance the UK as a world leader in a wide range of academic research areas, including engineering, materials, physics, chemistry, biology and medicine.

纽卡斯尔大学（NCL）在学术领导的研究项目和大规模的战略举措，如EPSRC前沿奖多学科合作的历史（2013年10月至2019年6月，该研究涉及来自纽卡斯尔大学四所不同学校的研究人员）。NCL资本设备战略旨在确保长期长期使用设备和计算设施，支持我们从常规表征到最先进技术开发的各种研究需求。我们认识到，纽卡斯尔和广泛的N8合作大学之间的科学设备共享（英格兰北部八所研究密集型大学）带来了多重好处，包括提高利用率，加强合作，增强的可持续性和更广泛的设施提供给我们的研究人员。我们已经确定了所需的建议设备，以支持ESPRC材料优先领域的研究表征、功能材料、生命与健康工程、工程科学、水、合成生物学、再生医学和纳米医学。这将使NCL的多个研究小组以及学术和工业界的外部用户受益。它支持现有的EPSRC赠款组合，并带来新的能力，使新的科学发现。所需设备及其功能的简要概述如下：1）。由于AFM和微流体功能提供的精确力控制，与微流体集成的尖端AFM为单细胞生物学和纳米科学中令人兴奋的应用提供了前所未有的可能性。高分辨率和高精度成像和机械测量的能力支持一系列化学项目及其与生物学，物理学和化学工程的接口。它由纽卡斯尔现有的最先进的高分辨率表面化学分析技术进行补充。2）。光学相干断层扫描（OCT）使用红外光提供表面轮廓和表面下结构，提供比超声检查更高的分辨率和更快的图像。当与定制的空气脉冲系统一起使用时，OCT还可以表征材料的弹性特性，如生物膜和软组织。项目AFM和OCT连同现有的设施在纽卡斯尔允许映射的三维几何形状和机械特性从亚纳米到毫米尺度。该资产使软物质的物理和动力学特性的研究，促进在亚细胞，细胞和组织水平的疾病进展的调查，这将允许制定有效的疾病诊断和治疗策略。该资产获得的多尺度定量测量还可以提供构建医疗保健和水工程预测模型所需的数据集。因此，它可以支持海洋生物膜，生物废水处理，生物膜感染，组织工程，癌症研究和许多其他医疗保健相关应用的一系列学术和行业重点项目。这种多尺度的物理和机械表征能力可以通过纽卡斯尔先进的表面化学分析设施得到进一步加强，这将引起世界领先的材料表征研究。它将加强英国作为一个世界领导者在广泛的学术研究领域，包括工程，材料，物理，化学，生物和医学。

项目成果

Mechanosensing model of fibroblast cells adhered on a substrate with varying stiffness and thickness

• DOI: 10.1016/j.jmps.2022.105137
• 发表时间: 2022-11-21
• 期刊: JOURNAL OF THE MECHANICS AND PHYSICS OF SOLIDS
• 影响因子: 5.3
• 作者: Yang，Wenjian;Luo，Ma;Chen，Jinju
• 通讯作者: Chen，Jinju

Slippery Liquid-Like Solid Surfaces with Promising Antibiofilm Performance under Both Static and Flow Conditions.

• DOI: 10.1021/acsami.1c14533
• 发表时间: 2022-02-09
• 期刊: ACS APPLIED MATERIALS & INTERFACES
• 影响因子: 9.5
• 作者: Zhu, Yufeng;McHale, Glen;Dawson, Jack;Armstrong, Steven;Wells, Gary;Han, Rui;Liu, Hongzhong;Vollmer, Waldemar;Stoodley, Paul;Jakubovics, Nicholas;Chen, Jinju
• 通讯作者: Chen, Jinju

Simultaneous determination of the mechanical properties and turgor of a single bacterial cell using atomic force microscopy

使用原子力显微镜同时测定单个细菌细胞的机械特性和膨胀度

• DOI: 10.1039/d2nr02577a
• 发表时间: 2022
• 期刊: Nanoscale
• 影响因子: 6.7
• 作者: Han R

Modelling the combined effect of surface roughness and topography on bacterial attachment

• DOI: 10.1016/j.jmst.2021.01.011
• 发表时间: 2021-08
• 期刊: Journal of Materials Science & Technology
• 影响因子: 10.9
• 作者: S. Chinnaraj;P. G. Jayathilake;Jack Dawson;Y. Ammar;J. Portoles;N. Jakubovics;Jinju Chen

Revealing the nanoindentation response of a single cell using a 3D structural finite element model

• DOI: 10.1557/s43578-020-00004-5
• 发表时间: 2021-01
• 期刊: Journal of Materials Research
• 影响因子: 2.7
• 作者: Wenjian Yang;D. Lacroix;L. P. Tan;Jinju Chen