MULTISCALE CHARACTERIZATION OF MONOSYNAPTIC CIRCUITS IN THE BRAIN

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 一种基于狂犬病病毒的新方法将被用于(1)标记神经元和与这些神经元相关的突触前终末，以及(2)确定是否所有撞击到已识别的突触后神经元的突触前终末都被该方法跨突触标记。NCMIR在相关光学显微镜(LM)和电子显微镜(EM)的标记化学方面的发展将扩展到与该系统一起工作，从而能够对保存良好的标本中的标记末端和相关结构进行高分辨率评估。正在开发的产生多色EM图像的新方法的使用也将被探索，以使这些方法能够分层，以解决有关已识别的突触复合体中多种分子成分的详细分布的问题。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A new rabies virus-based method for mapping monosynaptic connections in neuronal circuits will be used to (1) label neurons and pre-synaptic terminals associated with those neurons and (2) determine if all pre-synaptic terminals impinging on the identified postsynaptic neuron are trans-synaptically marked by this approach. NCMIR developments in labeling chemistry for correlated light microscopy (LM) and electron microscopy (EM) will be extended to work with this system enabling high-resolution assessment of labeled terminals and associated structures in well-preserved specimens. The use of the new method being developed to produce ¿¿multicolor EM¿¿ images will also be explored to enable layering of these methods to address questions regarding detailed distribution of multiple molecular constituents in identified synaptic complexes.