MULTISCALE CHARACTERIZATION OF MONOSYNAPTIC CIRCUITS IN THE BRAIN

大脑单突触回路的多尺度表征

• 批准号: 8169619
• 负责人: EDWARD M CALLAWAY
• 金额: $ 1.19万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169619
• 关键词: Address; Brain; Chemistry; Complex; Computer Retrieval of Information on Scientific Projects Database; Development; Electron Microscopy; Funding; Grant; Image; Institution; Label; Maps; Methods; Molecular; Neurons; Presynaptic Terminals; Rabies virus; Research; Research Personnel; Resolution; Resources; Source; Specimen; Structure; Synapses; System; United States National Institutes of Health; Work; base; light microscopy; postsynaptic

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A new rabies virus-based method for mapping monosynaptic connections in neuronal circuits will be used to (1) label neurons and pre-synaptic terminals associated with those neurons and (2) determine if all pre-synaptic terminals impinging on the identified postsynaptic neuron are trans-synaptically marked by this approach. NCMIR developments in labeling chemistry for correlated light microscopy (LM) and electron microscopy (EM) will be extended to work with this system enabling high-resolution assessment of labeled terminals and associated structures in well-preserved specimens. The use of the new method being developed to produce ¿¿multicolor EM¿¿ images will also be explored to enable layering of these methods to address questions regarding detailed distribution of multiple molecular constituents in identified synaptic complexes.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 一种基于狂犬病病毒的新方法用于绘制神经元回路中的单突触连接，该方法将用于（1）标记神经元和与这些神经元相关的突触前末梢，以及（2）确定撞击已识别的突触后神经元的所有突触前末梢是否都通过这种方法进行跨突触标记。 NCMIR 在相关光学显微镜 (LM) 和电子显微镜 (EM) 标记化学方面的发展将扩展到与该系统配合使用，从而能够对保存完好的标本中的标记末端和相关结构进行高分辨率评估。还将探索使用正在开发的产生“多色电子显微镜”图像的新方法，使这些方法能够分层，以解决有关已识别的突触复合体中多种分子成分的详细分布的问题。