BIOLOGICAL MARKERS OF ETHYLENE OXIDE & STYRENE EXPOSURE

环氧乙烷的生物标志物

• 批准号: 3548835
• 负责人: FREDERICA P PERERA
• 金额: $ 20.75万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-15 至 1989-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3548835
• 关键词: DNA; DNA repair; air sampling /monitoring; binding proteins; blood chemistry; chemical carcinogen; chemical conjugate; cytogenetics; environmental contamination; environmental toxicology; ethylene oxide; extrachromosomal DNA; gas chromatography; genetic markers; hospital personnel; human subject; immunologic techniques; industry; infrared spectrometry; lymphocyte; mutagen testing; occupational hazard; polystyrenes

We propose to conduct two related molecular epidemiological studies to characterize and validate five different markers of biologically effective dose in worker populations with well-characterized and substantial exposure to the mutagenic and carcinogenic chemicals: ethylene oxide (EtO) and styrene. The markers will include DNA- (lymphocyte) and protein- (hemoglobin) adducts, sister chromatid exchange, micronuclei in lymphocytes and unscheduled DNA synthesis. This battery of genetic markers will all be performed on the same blood sample to provide comparable data. This study will provide information on three different mechanisms involved in carcinogenesis: covalent binding to DNA, chromosomal damage, and DNA repair. The markers used will also provide comparative information on long-term exposure (refected by lymphocyte DNA0 and that incurred during the last four months (hemoglobin). Extensive workplace and personal monitoring will be carried out to fully characterize current ambient exposures; these data will be related to assay measurements. Data on historical exposures to these chemicals, on other relevant exposures and on potential confounding variables will be gathered by questionnaire. Although prior human studies have demonstrated that EtO and styrene can induce the biological effects to be measured here, most have been limited with respect to exposrue data and control of potential confounding variables. Moreover, none has applied a combination or "battery" of methods. This approach has the advantage of being cost-effective since major costs are incurred during the collection and initial processing of samples and in obtaining exposure and questionnaire data. Running multiple assays on the same samples allows significant cost-saving and permits evaluation of the relative cost-effectiveness of each method.

我们建议进行两项相关的分子流行病学研究， 表征并验证生物有效性的五种不同标记物 具有明确特征和大量接触的工人群体中的剂量 致突变和致癌化学品：环氧乙烷（EtO）和 苯乙烯。 标记物将包括DNA-（淋巴细胞）和蛋白质- （血红蛋白）加合物、姐妹染色单体交换、淋巴细胞微核 和非程序性DNA合成 这些基因标记 在相同的血液样本上进行，以提供可比数据。 这项研究将提供有关三种不同机制的信息 在致癌作用中：与DNA的共价结合、染色体损伤和DNA 修复. 所使用的标记还将提供以下方面的比较信息： 长期暴露（淋巴细胞DNA 0和 4个月（血红蛋白） 广泛的工作场所和个人 将进行监测，以充分表征当前环境 暴露量;这些数据将与含量测定相关。 数据 这些化学品的历史接触情况、其他相关接触情况以及 将通过问卷调查收集潜在的混杂变量。 尽管先前的人体研究已经证明环氧乙烷和苯乙烯可以 导致生物效应在这里被测量，大多数都是有限的 关于随机数据和潜在混杂控制 变量 此外，没有一个国家采用了以下措施的组合或"组合"： 方法. 这种方法具有成本效益的优点，因为 主要费用发生在收集和初步处理 样本和获取接触和问卷调查数据。 运行多个 在相同样品上的测定允许显著的成本节约，并且允许 评估每种方法的相对成本效益。

项目成果

Biomarkers in styrene-exposed boatbuilders.

接触苯乙烯的造船厂的生物标志物。

• DOI: 10.1016/0165-1218(91)90071-s
• 发表时间: 1991
• 期刊: Mutation research
• 作者: Brenner,DD;Jeffrey,AM;Latriano,L;Wazneh,L;Warburton,D;Toor,M;Pero,RW;Andrews,LR;Walles,S;Perera,FP
• 通讯作者: Perera,FP

Biologic markers in ethylene oxide-exposed workers and controls.

接触环氧乙烷的工人和对照人员的生物标记。

• DOI: 10.1016/0027-5107(91)90098-9
• 发表时间: 1991
• 期刊: Mutation research
• 作者: Mayer,J;Warburton,D;Jeffrey,AM;Pero,R;Walles,S;Andrews,L;Toor,M;Latriano,L;Wazneh,L;Tang,D
• 通讯作者: Tang,D