High Resolution High Throughput Mass Spectrometry to Characterise Materials, Chemicals, and BioCatalysts

用于表征材料、化学品和生物催化剂的高分辨率高通量质谱

• 批准号: EP/T019328/1
• 负责人: Perdita Barran
• 金额: $ 140.09万
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FT019328%2F1
• 关键词: Resolution; Throughput; Mass; Spectrometry; Characterise

This proposal will equip UoM new equipment that will enable high throughput high resolution ion mobility mass spectrometry analysis. This bid will increase capacity for analysis and extend our capacity to measure a wide range of analytes. Specialist equipment will be used to measure the mass and the shape of a large number of newly synthesised chemicals. In the simplest terms possible, mass spectrometry measures the movement of ions in a vacuum to give mass and ion mobility mass spectrometry measures the movement of ions in a gas to give shape. If we measure mass very accurately we can work out what atoms a molecule is made of and if we measure the shape very accurately we can work out how the atoms are arranged. So for a large range of different molecules we will be able to tell what we have made and how much we have made. The PI has been making ion mobility mass spectrometers and using ion mobility mass spectrometry for over 20 years. She has pioneered its use in many areas of chemistry and in this proposal we plan to do it even faster and with more accuracy. This equipment will be located with the Manchester Institute for Biotechnology in the Michael Barber Centre for Collaborative Mass Spectrometry. It will be used by research teams at the University of Manchester as well as by external users from both academia and instrument. We will use this state of the art instrumentation to underpin investments by EPRSC in the Future Biomanufacturing Research hub and the Prosperity Partnership with AstraZeneca as well as other programs in materials and catalysis. This new capability will consist of two mass spectrometers coupled to novel ionisation sources. A State-of-the-art Cyclic IMS QTof MS instrument will provide unique enhanced analytical capabilities for molecular identification and structural characterisation of complex mixtures complete with full ion mobility data. A second "work-horse" DESI interfaced Synapt XS instrument will provide broader research access and wider application, and act as an initial screening platform prior to Cyclic Structural work for rapid mass spectrometry analysis. The ionisation sources will enable analysis with minimal sample preparation both directly from surfaces via Desorption Electrospray ionisation and with microfluidic droplet dispensing to provide a unique ultra-high-throughput (uHTP) screening capability. This will provide mass and structural data for a range of chemical analytes from small molecules to polymers, supramolecular compounds to biopharmaceuticals.

这项提议将为UOM配备新的设备，使其能够进行高通量、高分辨率的离子迁移质谱分析。此次投标将增加分析能力，并扩大我们测量各种分析物的能力。将使用专门的设备来测量大量新合成的化学品的质量和形状。在最简单的术语中，质谱仪测量离子在真空中的运动以获得质量，而离子迁移率质谱仪测量离子在气体中的运动以获得形状。如果我们非常准确地测量质量，我们就可以计算出分子是由什么原子组成的，如果我们非常精确地测量形状，我们就可以计算出原子是如何排列的。因此，对于大范围的不同分子，我们将能够分辨出我们制造了什么以及我们制造了多少。PI制造离子迁移质谱计和使用离子迁移质谱计已有20多年的历史。她率先在化学的许多领域使用它，在这个提案中，我们计划做得更快、更准确。该设备将与曼彻斯特生物技术研究所一起安装在迈克尔巴伯合作质谱学中心。它将由曼彻斯特大学的研究团队以及来自学术界和仪器的外部用户使用。我们将使用这种最先进的仪器来支持EPRSC对未来生物制造研究中心和与阿斯利康的繁荣伙伴关系以及其他材料和催化项目的投资。这一新能力将包括两个质谱仪与新的电离源耦合。最先进的循环IMS QTof MS仪器将为复杂混合物的分子鉴定和结构表征提供独特的增强分析能力，并提供完整的离子迁移率数据。第二个与Synapt XS仪器接口的“工作马”将提供更广泛的研究途径和更广泛的应用，并作为循环结构工作之前的初始筛选平台，用于快速质谱分析。电离源将通过解吸电喷雾电离直接从表面制备最少的样品即可进行分析，并通过微流控液滴分配提供独特的超高通量(UHTP)筛选能力。这将为从小分子到聚合物、超分子化合物到生物制药的一系列化学分析物提供质量和结构数据。

项目成果

Galactose Oxidase Enables Modular Assembly of Conjugates from Native Antibodies with High Drug-to-Antibody Ratios.

• DOI: 10.1002/cssc.202102592
• 发表时间: 2022-05-06
• 期刊: ChemSusChem
• 影响因子: 8.4

Advanced Fragmentation Methods in Biomolecular Mass Spectrometry - Probing Primary and Higher Order Structure with Electrons, Photons and Surfaces

生物分子质谱中的高级碎裂方法 - 用电子、光子和表面探测初级和高阶结构

• DOI: 10.1039/9781839161056-00235
• 发表时间: 2020
• 作者: Black R

Characterization of native protein structure with ion mobility mass spectrometry, multiplexed fragmentation strategies and multivariant analysis

• DOI: 10.1016/j.ijms.2021.116588
• 发表时间: 2021-03-26
• 期刊: INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
• 影响因子: 1.8
• 作者: Black, Rachelle;Barkhanskiy, Alexey;Barran, Perdita E.
• 通讯作者: Barran, Perdita E.

Ion Mobility Mass Spectrometry (IM-MS) for Structural Biology: Insights Gained by Measuring Mass, Charge, and Collision Cross Section.

• DOI: 10.1021/acs.chemrev.2c00600
• 发表时间: 2023-03-22
• 期刊: CHEMICAL REVIEWS
• 影响因子: 62.1
• 作者: Christofi, Emilia;Barran, Perdita
• 通讯作者: Barran, Perdita

Hexanuclear Ln6 L6 Complex Formation by Using an Unsymmetric Ligand.

使用不对称配体形成六核 Ln6 L6 络合物。

• DOI: 10.1002/chem.202302497
• 发表时间: 2023
• 期刊: Chemistry (Weinheim an der Bergstrasse, Germany)
• 作者: Bell DJ