MRI: Acquisition of a High-throughput/High Resolution Orbitrap Mass Spectrometer to Enable Multidisciplinary Research and Education in the South-East Wisconsin Area

MRI：购买高通量/高分辨率 Orbitrap 质谱仪，以实现威斯康星州东南部地区的多学科研究和教育

• 批准号: 2214658
• 负责人: Shama Mirza
• 金额: $ 53.87万
• 依托单位: University of Wisconsin-Milwaukee
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-10-01 至 2025-09-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2214658&HistoricalAwards=false
• 关键词: MRI; Acquisition; throughput; Resolution; Orbitrap

An award is made to the University of Wisconsin-Milwaukee (UWM) to acquire a Thermo Scientific Orbitrap Exploris Mass Spectrometer. This new mass spectrometry (MS) instrument enhances the core analytical infrastructure at UWM creating new research and learning experiences at all levels. It provides opportunities to students who are first-generation and underrepresented in the STEM fields, outreach programs for local K-12 students, science teachers and the public. The enhanced MS infrastructure will create new research and learning experiences and will expand the existing MS curriculum. It includes hands-on training with advanced research instrumentation to undergraduate, graduate, and postdoctoral researchers. The use of this instrument will inspire STEM interest among students, including women and underrepresented individuals, who are doing research in the labs of the project faculty. UWM’s MS facility has a strong record of industrial collaborations, which will be expanded with the new MS, by providing expert support in applications development, experimental design, and data analysis. Collaborations also play a vital role in financial sustainability of MS facility operations, diversify the scope of applications and projects, and give students hands-on experience working with industrial scientists and commercial problems. Students gaining this experience will be highly competent in their future jobs in a variety of chemistry-related industries.The new MS instrumentation will enable a diverse cadre of early-career and senior researchers at the UWM and regional universities to perform advanced proteomics, metabolomics, and high-resolution mass spectrometry analysis. The instrument will be used for the comprehensive characterization of small compounds like organic molecules and metabolites as well as large molecules like proteins, peptides and oligonucleotides with a high degree of precision and accuracy. This instrument will have immediate impacts on several ongoing projects such as (1) understanding the role of acid ceramidase in ceramide metabolism pathway; (2) identifying proteins and their PTMs that are secreted by the type IX secretion system of Flavobacterium johnsoniae; and (3) identifying and quantifying phosphorylated and acetylated proteins that are targeted by the stress response pathways in yeast and metazoan cells. In addition, this instrument will advance the cross-disciplinary research pursued by scientific teams at the UWM and other local academic and industrial institutions in the fields of biochemistry, microbiology, neurochemistry, environmental chemistry, and biomaterial engineering.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

威斯康星大学 - 米尔沃基大学（UWM）颁发了奖励，以获取Thermo Scientific Orbitrap Exploris质谱仪。这种新的质谱仪器（MS）仪器增强了UWM的核心分析基础架构，从而在各个级别创造了新的研究和学习经验。它为在STEM领域的第一代学生，为本地K-12学生，科学老师和公众提供的外展计划提供了机会。增强的MS基础架构将创造新的研究和学习经验，并将扩大现有的MS课程。它包括针对本科，研究生和博士后研究人员的高级研究工具的动手培训。该工具的使用将激发学生在包括妇女和代表性不足的个人中进行研究的学生的兴趣，这些人正在项目教师的实验室进行研究。 UWM的MS工厂具有较强的记录合作，在MS设施运营的财务可持续性，使应用程序和项目的范围多样化，并为学生提供与工业科学家和商业问题一起工作的动手经验。学生在各种与化学相关的行业中都能获得这一经验。新的MS仪器将使UWM和区域大学的早期职业生涯和高级研究人员能够执行先进的蛋白质组学，代谢组学和高分辨率质谱分析。该仪器将用于综合特征，例如有机分子和代谢产物，以及蛋白质，肽和寡核苷酸等大分子，具有高度的精度和准确性。该仪器将对几个正在进行的项目产生直接影响，例如（1）了解酸性神经酶在神经酰胺代谢途径中的作用； （2）鉴定由约翰逊植物的IX型分泌系统分泌的蛋白质及其PTM； （3）鉴定和量化磷酸化和乙酰化蛋白，这些蛋白质是由酵母和后生细胞中的应激反应途径靶向的。此外，该工具还将推进UWM和其他本地学术和工业工具在生物化学，微生物学，神经化学，环境化学，环境化学和生物材料工程领域的跨学科研究。该奖项通过评估委员会的支持和广泛的基础，反映了NSF的法规及其对支持的支持。