MRI: Acquisition of a High-throughput/High Resolution Orbitrap Mass Spectrometer to Enable Multidisciplinary Research and Education in the South-East Wisconsin Area

威斯康星大学密尔沃基分校(UWM)获得了一台热科学轨道探测质谱仪。这一新的质谱仪增强了威斯康星大学的核心分析基础设施，在所有级别创造了新的研究和学习体验。它为STEM领域的第一代和代表性不足的学生提供机会，为当地K-12学生、科学教师和公众提供外联计划。增强的MS基础设施将创造新的研究和学习体验，并将扩展现有的MS课程。它包括使用先进的研究仪器对本科生、研究生和博士后研究人员进行动手培训。该仪器的使用将激发学生对STEM的兴趣，包括在项目教员实验室进行研究的女性和代表性不足的个人。UWM的MS设施在行业合作方面有着良好的记录，通过在应用程序开发、实验设计和数据分析方面提供专家支持，这种合作将随着新的MS而得到扩展。协作在MS设施运营的财务可持续性方面也发挥着至关重要的作用，使应用程序和项目的范围多样化，并为学生提供与工业科学家和商业问题合作的实践经验。获得这一经验的学生将在未来的各种化学相关行业的工作中非常胜任。新的MS仪器将使华盛顿大学和地区性大学的一批职业早期和高级研究人员能够进行高级蛋白质组学、代谢组学和高分辨率质谱学分析。该仪器将用于有机分子和代谢物等小分子以及蛋白质、多肽和寡核苷酸等大分子的高精密度和准确性的综合表征。该仪器将对正在进行的几个项目产生立竿见影的影响，如：(1)了解酸性神经酰胺酶在神经酰胺代谢途径中的作用；(2)识别由强生黄杆菌IX型分泌系统分泌的蛋白质及其PTM；以及(3)识别和定量酵母和后生动物细胞中应激反应途径靶标的磷酸化和乙酰化蛋白质。此外，该仪器将推动华盛顿大学和其他地方学术和工业机构的科学团队在生物化学、微生物学、神经化学、环境化学和生物材料工程领域进行的跨学科研究。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

An award is made to the University of Wisconsin-Milwaukee (UWM) to acquire a Thermo Scientific Orbitrap Exploris Mass Spectrometer. This new mass spectrometry (MS) instrument enhances the core analytical infrastructure at UWM creating new research and learning experiences at all levels. It provides opportunities to students who are first-generation and underrepresented in the STEM fields, outreach programs for local K-12 students, science teachers and the public. The enhanced MS infrastructure will create new research and learning experiences and will expand the existing MS curriculum. It includes hands-on training with advanced research instrumentation to undergraduate, graduate, and postdoctoral researchers. The use of this instrument will inspire STEM interest among students, including women and underrepresented individuals, who are doing research in the labs of the project faculty. UWM’s MS facility has a strong record of industrial collaborations, which will be expanded with the new MS, by providing expert support in applications development, experimental design, and data analysis. Collaborations also play a vital role in financial sustainability of MS facility operations, diversify the scope of applications and projects, and give students hands-on experience working with industrial scientists and commercial problems. Students gaining this experience will be highly competent in their future jobs in a variety of chemistry-related industries.The new MS instrumentation will enable a diverse cadre of early-career and senior researchers at the UWM and regional universities to perform advanced proteomics, metabolomics, and high-resolution mass spectrometry analysis. The instrument will be used for the comprehensive characterization of small compounds like organic molecules and metabolites as well as large molecules like proteins, peptides and oligonucleotides with a high degree of precision and accuracy. This instrument will have immediate impacts on several ongoing projects such as (1) understanding the role of acid ceramidase in ceramide metabolism pathway; (2) identifying proteins and their PTMs that are secreted by the type IX secretion system of Flavobacterium johnsoniae; and (3) identifying and quantifying phosphorylated and acetylated proteins that are targeted by the stress response pathways in yeast and metazoan cells. In addition, this instrument will advance the cross-disciplinary research pursued by scientific teams at the UWM and other local academic and industrial institutions in the fields of biochemistry, microbiology, neurochemistry, environmental chemistry, and biomaterial engineering.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.