MRI: Acquisition of a High-throughput/High Resolution Orbitrap Mass Spectrometer to Enable Multidisciplinary Research and Education in the South-East Wisconsin Area

威斯康星大学密尔沃基分校（UWM）获得了一份合同，以获得一台热科学轨道探测器质谱仪。这种新的质谱（MS）仪器增强了UWM的核心分析基础设施，在各个层面创造了新的研究和学习经验。它为STEM领域中代表性不足的第一代学生提供了机会，为当地K-12学生、科学教师和公众提供了拓展项目。增强的MS基础设施将创造新的研究和学习体验，并将扩展现有的MS课程。它包括为本科生、研究生和博士后研究人员提供先进研究仪器的实践培训。该工具的使用将激发在项目教师实验室进行研究的学生（包括女性和代表性不足的个人）对STEM的兴趣。UWM的MS设施拥有强大的工业合作记录，将通过在应用程序开发，实验设计和数据分析方面提供专家支持，扩展新的MS。合作在MS设施运营的财务可持续性方面也发挥着至关重要的作用，使应用和项目的范围多样化，并为学生提供与工业科学家和商业问题合作的实践经验。获得这种经验的学生将在未来各种化学相关行业的工作中具有很强的竞争力。新的质谱仪器将使UWM和地区大学的各种早期职业和高级研究人员能够进行先进的蛋白质组学，代谢组学和高分辨率质谱分析。该仪器将用于有机分子和代谢物等小化合物以及蛋白质、多肽和寡核苷酸等大分子的综合表征，具有很高的精度和准确性。该仪器将对几个正在进行的项目产生直接影响，如：(1)了解酸性神经酰胺酶在神经酰胺代谢途径中的作用；(2)鉴定琼氏黄杆菌IX型分泌系统分泌的蛋白及其PTMs；(3)鉴定和定量酵母和后生动物细胞中应激反应途径靶向的磷酸化和乙酰化蛋白。此外，该仪器还将推动澳大及其他本地学术和工业机构的科研团队在生物化学、微生物学、神经化学、环境化学和生物材料工程等领域的跨学科研究。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

An award is made to the University of Wisconsin-Milwaukee (UWM) to acquire a Thermo Scientific Orbitrap Exploris Mass Spectrometer. This new mass spectrometry (MS) instrument enhances the core analytical infrastructure at UWM creating new research and learning experiences at all levels. It provides opportunities to students who are first-generation and underrepresented in the STEM fields, outreach programs for local K-12 students, science teachers and the public. The enhanced MS infrastructure will create new research and learning experiences and will expand the existing MS curriculum. It includes hands-on training with advanced research instrumentation to undergraduate, graduate, and postdoctoral researchers. The use of this instrument will inspire STEM interest among students, including women and underrepresented individuals, who are doing research in the labs of the project faculty. UWM’s MS facility has a strong record of industrial collaborations, which will be expanded with the new MS, by providing expert support in applications development, experimental design, and data analysis. Collaborations also play a vital role in financial sustainability of MS facility operations, diversify the scope of applications and projects, and give students hands-on experience working with industrial scientists and commercial problems. Students gaining this experience will be highly competent in their future jobs in a variety of chemistry-related industries.The new MS instrumentation will enable a diverse cadre of early-career and senior researchers at the UWM and regional universities to perform advanced proteomics, metabolomics, and high-resolution mass spectrometry analysis. The instrument will be used for the comprehensive characterization of small compounds like organic molecules and metabolites as well as large molecules like proteins, peptides and oligonucleotides with a high degree of precision and accuracy. This instrument will have immediate impacts on several ongoing projects such as (1) understanding the role of acid ceramidase in ceramide metabolism pathway; (2) identifying proteins and their PTMs that are secreted by the type IX secretion system of Flavobacterium johnsoniae; and (3) identifying and quantifying phosphorylated and acetylated proteins that are targeted by the stress response pathways in yeast and metazoan cells. In addition, this instrument will advance the cross-disciplinary research pursued by scientific teams at the UWM and other local academic and industrial institutions in the fields of biochemistry, microbiology, neurochemistry, environmental chemistry, and biomaterial engineering.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.